Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar 26:32:148.
doi: 10.11604/pamj.2019.32.148.16314. eCollection 2019.

Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples

Daniel David Osiagwu  1 , Alfred Ederialo Azenabor  1 , Abimbola Akinduro Osijirin  2 , Philomina Ikuoyah Awopetu  1 , Folasade Ruth Oyegbami  1
Affiliations

Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples

Daniel David Osiagwu et al. Pan Afr Med J. .

Abstract

Introduction: inflammatory cytokines have been associated with various cancers, including cervical cancers. Interpreting cytokine expression in liquid based cervical samples is quite challenging. This study is aimed at evaluating the levels of interleukin 8 and 10 in liquid based cervical samples.

Methods: this is a descriptive analytical study carried out on eighty five (85) subjects aged between 23 and 68 years. Cervical samples were collected in liquid based medium and smears later examined after staining with Papanicolaou technique. These were categorized into low grade intra-epithelial lesion/malignancy, high grade intraepithelial lesion/malignancy according to the degree of dyskaryosis. Concentrations of interleukin 8 and interleukin 10 in the samples were determined by enzyme linked immunosorbent assay.

Results: the mean age, standard deviation (SD) of the study subjects were 40.6 (7.8) years. A total number of 79 females (92.9%) were negative for intra-epithelial lesion/malignancy (NILM), while 4 (4.71%) and 2 (2.35%) were positive for low grade intra-epithelial lesion/malignancy (LILM) and high grade intra-epithelial lesion (HILM) respectively. While mean levels of interleukin 8 increased with the degree of malignancy, (107.27 ± 11.88pg/ml) in LILM, (114.80 ± 2.12pg/ml) in HILM when compared with NILM (88.39 ± 18.06pg/ml), (f = 0.700, p = 0.018); the mean levels of interleukin 10 was comparable between these groups (p ≥ 0.05). Pearson correlation coefficient analysis showed a negative association between interleukin 8 and interleukin 10 (r = -1.999, p = 0.000) in LILM.

Conclusion: interleukin 8 cytokines in cervical cancer is associated with the degree of malignancy. Possible anti-inflammatory effect of interleukin 10 was not observed.

Keywords: Cytokines; cervical cancer; inflammation.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
negative intraepithelial lesion/malignancy on an inflammatory background
Figure 2
Figure 2
low grade intraepithelial lesion/malignancy on atrophic smear
Figure 3
Figure 3
high grade intraepithelial lesion/malignancy on atrophic smear
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Murooka TT, Ward SE, Fish EN. Chemokines and Cancer. Cancer Treat Res. 2005;126:15–44. - PubMed
    1. Zarogoulidis P, Yarmus L, Zarogoulidis K. Interleukin-6 cytokine: a multifunctional glycoprotein for cancer. Immunome Res. 2013 Aug 12;9(62):16635. - PMC - PubMed
    1. Christopher Dolinsky. “Cervical Cancer: The Basics.”. Abramson Cancer Center of the University of Pennsylvania; 2007. OncoLink.
    1. Lowe JS, Anderson Peter G, Stevens Alan. Stevens & Lowe's human histology. Fourth edition. Philadelphia, PA: Elsevier/Mosby; 2015.
    1. Doorbar J. Molecular biology of human papillomavirus infection and cervical cancer. Clinical Science. 2006;110(5):525–541. - PubMed