Blood flow velocity in pulmonary microvessels of bullfrog

Abstract

Flow velocity in the pulmonary microvessels of the exposed lung of bullfrogs was measured by means of a laser Doppler microscope of an oblique backward mode, together with a signal-analyzing system having a time sharing circuit triggered by the R-wave of the ECG. By these means, measurements of the changes of flow velocity contour in the cardiac cycle were made. Flow velocity was clearly pulsatile in response to cardiac cycles in all microvessels including capillaries. Flow velocities in the arteriole and venule consistently decreased for a short period after the R-wave (84 +/- 33 msec (mean +/- SD) in the arteriole and 130 +/- 31 msec in the venule, respectively) and rapidly increased up to a maximicronm value. The mean flow velocities in arterioles (diameter 50 +/- 17 micron) and venules (39 +/- 9 micronm) were 2.29 +/- 0.32 and 2.30 +/- 0.27 mm/sec. The amplitudes of pulsatile flow in these vessels were 0.83 +/- 0.31 and 0.63 +/- 0.16 mm/sec, respectively. In the capillary the times from the R-wave to the minimicronm and maximum values were variable. In some cases the velocity gradually increased without first decreasing and the increase sharply accelerated a certain time after the R-wave. The mean velocity in the pulmonary capillary and the amplitude of the pulsatile flow ere 1.78 +/- 0.31 and 0.37 +/- 0.12 mm/sec, resepctively. The ratios of the pulsatile amplitude to the mean velocity in the pulmonary capillary, venule and arteriole averaged 0.21, and 0.36, respectively.