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Published Erratum
. 2019 Jul 26:17:225-229.
doi: 10.1016/j.isci.2019.06.017. Epub 2019 Jul 12.

Modulation of TNFα Activity by the microRNA Let-7 Coordinates Zebrafish Heart Regeneration

Ashley M Smith, Christina A Dykeman, Benjamin L King, Viravuth P Yin
Published Erratum

Modulation of TNFα Activity by the microRNA Let-7 Coordinates Zebrafish Heart Regeneration

Ashley M Smith et al. iScience. .

Retraction in

No abstract available

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Figures

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Figure 2. Let-7 Depletion Results in Defects in Wound Closure (Corrected) (A) Schematic of locked-nucleic-acid (LNA) microinjection paradigm to deplete let-7 expression. (B) Real-time qPCR analyses show that LNA treatment results in significant knockdown of mature let-7 expression at 3 dpa, in comparison to scrambled control LNA oligonucleotides. Values are means ± SE. *p < 0.05. (n = 3). (C) Representative images of tcf21:Dsred expression in scrambled LNA control and LNA-let-7 treated hearts at 3, 14, and 21 dpa showing defects in wound closure. Scale bar represents 50 μm. Brackets indicate approximate resection plane. (D) Quantification of tcf21:Dsred expression levels within the resection zone. Values are means ± SE. *p < 0.05. (n = 6); IP, intraperitoneal; dpa, days post-amputation; NS, not significant.
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Figure 2. Let-7 Depletion Results in Defects in Wound Closure (Original)
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Figure 3. Let-7 Activity Is Required for Heart Regeneration (Corrected) (A) Representative images showing proliferating CMs in scrambled LNA control and LNA-let-7-treated fish. Arrowheads indicate Mef2+PCNA+ cells at the injury zone (n = 7). (B) CM proliferation indices are suppressed by 68% and 44% in LNA-let-7 hearts at 3 and 7 dpa, respectively, when compared to scrambled LNA control treatment. Values are means ± SE. *p < 0.05. (C) Representative images of hematoxylin and eosin staining of 14 dpa scrambled LNA control and LNA-let-7-treated hearts reveal a spectrum of heart regeneration defects, categorized as normal, mild, and severe. (N = 12). Dashed lines mark approximate resection plane; dpa, days post-amputation. (D) Representative images of AFOG stains of scrambled LNA control and LNA-let-7 administered hearts reveal more collagen and fibrin tissue within the injury zone. (N = 10). Brackets mark the injury zone. Brown, muscle; red, fibrin; blue, collagen.
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Figure 3. Let-7 Activity Is Required for Heart Regeneration (Original)
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Figure 4. CM Dedifferentiation Is Normal under Conditions of Decreased let-7 Activity (Corrected) (A) Representative transmission electron microscope images of remote and injury zones of resected 7-dpa hearts from scrambled control and LNA-let-7-treated hearts. Brackets mark intact CM sarcomeres in a remote zone. By contrast, asterisks highlight disorganized and dedifferentiated myosin bundles at the injury zone (n = 4). Scale bar represents 500 nm. (B) Representative images showing activation of Tg(gata4:GFP) expression in the primordial muscle layer in scrambled control and LNA-let-7-treated hearts at 7 dpa. Brackets in upper panels show approximate resection injury plane. Arrowheads in magnified bottom panels show gata4:GFP expression in both scrambled control and LNA-let-7-treated hearts. (n = 6). (C) Quantification of gata4:GFP expression levels at the injury border and remote zones show no differences between scrambled control and LNA-let-7-treated hearts. Values are means ± SE. *p < 0.05. (N = 6).
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Figure 4. CM Dedifferentiation Is Normal under Conditions of Decreased let-7 Activity (Original)
See this image and copyright information in PMC

Erratum for

Comment in

  • Findings of Research Misconduct.
    [No authors listed] [No authors listed] Fed Regist. 2021 Aug 19;86(158):46706-46707. Fed Regist. 2021. PMID: 34446974 Free PMC article. No abstract available.

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