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. 2019 Jul 1;11(1):e2019041.
doi: 10.4084/MJHID.2019.041. eCollection 2019.

Prevalence of bla TEM, bla SHV, and bla CTX-M Genes among ESBL-Producing Klebsiella pneumoniae and Escherichia coli Isolated from Thalassemia Patients in Erbil, Iraq

Ahmad Hamad Pishtiwan  1 , Khalil Mustafa Khadija  1
Affiliations

Prevalence of bla TEM, bla SHV, and bla CTX-M Genes among ESBL-Producing Klebsiella pneumoniae and Escherichia coli Isolated from Thalassemia Patients in Erbil, Iraq

Ahmad Hamad Pishtiwan et al. Mediterr J Hematol Infect Dis. .

Abstract

Background: Due to the recent appearance of organisms that are resistant to several drugs (multidrug-resistant) like Enterobacteriaceae that produce extended-spectrum β-lactamase (ESBL, concerns have remarkably increased regarding the suitable treatment of infections. The present study was an investigation into ESBL molecular characteristics among clinical isolates of Klebsiella pneumoniae and Escherichia coli resulting in urinary tract infections (UTIs) and their pattern of antimicrobial resistance in order to come up with helpful information on the epidemiology of these infections and risk factors accompanied with them.

Methods: In order to conduct the study, 20 K. pneumoniae and 48 E. coli were isolated and retrieved from thalassemia center in Erbil, Iraq during July 2016 and September 2016. The collected strains were analyzed and the profile of their antimicrobial susceptibility was specified. In order to spot β-lactamase genes (i.e. blaTEM, blaSHV, and blaCTX-M), polymerase chain reaction was conducted.

Results: The findings obtained from multiplex PCR assay showed that out of the collected strains of ESBL-producing E. coli, had 81% blaTEM, 16.2% blaSHV, and 32.4% blaCTX-M genes. Similarly, 64.7% blaTEM, 35.2% blaSHV, and 41.1% blaCTX-M genes existed in the isolates of K. pneumoniae. It was found that antibiotic resistance pattern of E. coli and K. pneumoniae isolates to 20 antibiotics varied widely. It was also concluded that the majority of the K. pneumoniae and E. coli isolates were multi-drug resistant (MDR). Moreover, 75% and 87.5% of respectively K. pneumoniae and E. coli isolates showed the MDR phenotypes.

Conclusion: TEM prevalence was high among other types of ESBLs. Over all, the most active antimicrobial agents in vitro remained to be the carbapenems.

Keywords: ESBL; Escherichia coli; Klebsiella pneumoniae; Thalassemia; blaTEM, blaSHV and blaCTX-M.

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Conflict of interest statement

Competing interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1
The overall incidence of ESBL genotypes in screening positive K. pneumoniae and E. coli isolates.
See this image and copyright information in PMC

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References

    1. Gaynes R, Edwards JR National Nosocomial Infections Surveillance System. Overview of nosocomial infections caused by gram-negative bacilli. Clin Infect Dis. 2005;41(6):848–54. doi: 10.1086/432803. - DOI - PubMed
    1. Ojdana D, Sacha P, Wieczorek P, Czaban S, Michalska A, Jaworowska J. The Occurrence of blaCTX-M, blaSHV, and blaTEM Genes in Extended-Spectrum β-Lactamase-Positive Strains of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis in Poland. Intern J Antibi. 2014:7. doi: 10.1155/2014/935842. Article ID 935842. - DOI
    1. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005;18(4):657–86. doi: 10.1128/CMR.18.4.657-686.2005. - DOI - PMC - PubMed
    1. Liao K, Chen Y, Wang M, Guo P, Yang Q, Ni Y, Yu Y, Hu B, Sun Z, Huang W. Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae causing intra-abdominal infections from 9 tertiary hospitals in China. Diagn Microbiol Infect Dis. 2017;87(1):45–48. doi: 10.1016/j.diagmicrobio.2016.10.007. - DOI - PubMed
    1. Malloy AM, Campos JM. Extended-spectrum beta-lactamases: a brief clinical update. Pediatr Infect Dis J. 2011;30(12):1092–3. doi: 10.1097/INF.0b013e31823c0e9d. - DOI - PubMed