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. 2019 Jul-Aug;9(4):396-409.

Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity

Earnest Oghenesuvwe Erhirhie  1 , Chika Ndubuisi Emeghebo  1 , Emmanuel Emeka Ilodigwe  1 , Daniel Lotanna Ajaghaku  2 , Blessing Ogechukwu Umeokoli  3 , Peter Maduabuchi Eze  4 , Kenneth Gerald Ngwoke  3 , Festus Basden Gerald Chiedu Okoye  3
Affiliations

Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity

Earnest Oghenesuvwe Erhirhie et al. Avicenna J Phytomed. 2019 Jul-Aug.

Abstract

Objective: Dryopteris filix-mas (D. filix-mas) (L.) Schott, (Dryopteridaceae) is used in traditional medicine, particularly in the Southern parts of Nigeria for the treatment of inflammation, rheumatoid arthritis, wounds and ulcers. In this study, we evaluated the anti-inflammatory activity of its ethanolic leaf extract and fractions.

Materials and methods: The ethanolic leaf extract and fractions were screened for anti-inflammatory properties using egg-albumin-induced paw edema, xylene-induced topical ear edema, formaldehyde-induced arthritis and ulcerogenic models. The ethyl acetate most promising vacuum liquid chromatography fraction (VLC-E7) was purified using size exclusion chromatography technique (Sephadex LH-20) and its structure was elucidated using nuclear magnetic resonance (NMR) and mass spectrometry. Total phenolic and flavonoid contents were also determined.

Results: From the study, ethyl acetate and butanol fractions elicited better anti-inflammatory activities in egg-albumin-induced paw edema, formaldehyde-induced arthritis and xylene-induced topical ear edema. The ethanol extract, ethyl acetate and butanol fractions were non-ulcerogenic at 200 and 400 mg/kg. The compound isolated from Sephadex fraction (SPH-E6) was quercetin-3-O-α-L-rhamnopyranoside.

Conclusion: Results of this study justify the ethnomedicinal use of D. filix-mas leaf for treatment of inflammation and rheumatoid arthritis. We suggest that D. filix-mas could be a prospective anti-inflammatory agent with no gastric irritation side effect, due to its bioactive component, quercetin-3-O-α-L-rhamnopyranoside.

Keywords: Anti-inflammatory; Dryopteris filix-mas; Non-ulcerogenic; Quercetin 3-O-α-L-rhamnopyranoside; Rheumatoid arthritis.

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Conflict of interest statement

The authors have declared that there is no conflict of interest.

Figures

Figure 1
Figure 1
HPLC chromatogram and UV spectrals of major compounds detected in the ethanolic leaf extract of D. filix mas. A= Quercitrin (Rt=25.33 min, 998.90) and B=Kmpf-3-O-rham (Rt=27.37, 996.14)
Figure 2
Figure 2
HPLC chromatogram and UV spectrals of major compounds detected in ethyl acetate fraction of D. filix mas A=Caffeoylglycolic acid (Rt=11.63 min, 996.33) and B=Quercitrin (Rt=21.32 min, 997.46)
Figure 3
Figure 3
HPLC chromatogram and UV spectrals of major compounds detected in VLC-E7 fraction. A=Quercitrin (Rt=20.69 min, 998.30) and B=Kmpf-3-O-rham (Rt=22.69 min, 998.40)
Figure 4
Figure 4
HPLC chromatogram and UV spectrals of major compounds detected in in SPH-E6 fraction. A= Quercitrin (Rt=21.07 min, 998.31) and B= Kmpf-3-O-rahm (Rt=22.65 min, 994.79)
Figure 5
Figure 5
Structure of isolated compound, Quercetin-3O-α-L-rhamnopyranoside
See this image and copyright information in PMC

References

    1. Alam B, Akter A, Parvin N, Pia RS, Akter S, Chowdhury J, Sifath-E-Jahan K, Haque E. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J Phytomed. 2013;3:112–125. - PMC - PubMed
    1. Ajaghaku DL, Ilodigwe EE, Okonta MJ, Ogue CO, Okafor MA, Igboeme SO. Mechanism of anti-inflammatory activity of the leaf extract and fractions of Millettia aboensis. IRJP. 2013;4:54–59.
    1. Ajaghaku DL, Obasi O, Umeokoli BO, Ogbuatu P, Nworu CS, Ilodigwe EE, Okoye FBC. In vitro and in vivo antioxidant potentials of Alchornea floribunda leaf extract, fractions and isolated bioactive compounds. Avicenna J Phytomed. 2017;7:80–92. - PMC - PubMed
    1. Azubike NC, Okwuosa CN, Achukwu PU, Maduka TC, Chike O. Acute toxicity and histopathological effects of crude aqueous extract of Jatropha curcas leaves in mice. Res J Med Plant. 2015;9:340–346.
    1. Bafor EE, Omokaro WO, Uwumarongie OH, Elvis-Offiah UB, Omoruyi O, Viegelmann CV, Edrada-Ebel R. Dryopteris filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. JOMPED. 2017;1:1–12.

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