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. 2019 Jun 12:2019:9130806.
doi: 10.1155/2019/9130806. eCollection 2019.

Study of Surface Structure Changes for Selected Ceramics Used in the CAD/CAM System on the Degree of Microbial Colonization, In Vitro Tests

Maciej Dobrzynski  1 , Magdalena Pajaczkowska  2 , Joanna Nowicka  2 , Aleksander Jaworski  1 , Piotr Kosior  1 , Maria Szymonowicz  3 , Piotr Kuropka  4 , Zbigniew Rybak  3 , Zdzislaw A Bogucki  5 , Jaroslaw Filipiak  6 , Sara Targonska  7 , Aneta Ciupa-Litwa  7 , Anna Han  7 , Rafal J Wiglusz  7
Affiliations

Study of Surface Structure Changes for Selected Ceramics Used in the CAD/CAM System on the Degree of Microbial Colonization, In Vitro Tests

Maciej Dobrzynski et al. Biomed Res Int. .

Abstract

In the article has been presented an analysis of susceptibility of selected dental materials, made in the CAD/CAM technology. The morphology and structural properties of selected dental materials and their composites were determined by using XRPD (X-ray powder diffraction) techniques, as well as the IR (infrared) spectroscopy. Moreover, an adhesion as well as development of biofilm by oral microorganisms has been studied. It has been shown that a degree of the biofilm development on the tested dental materials depended on microorganism genus and species. Streptococcus mutans has demonstrated the best adhesion to the tested materials in comparison with Candida albicans and Lactobacillus rhamnosus. However, the sintered materials such as IPS e.max® and the polished IPS e.max® have showed the best "anti-adhesive properties" in relation to S. mutans and L. rhamnosus that have not formed the biofilm on the polished IPS e.max® sample. Furthermore, S. mutans have not formed the biofilm on both surfaces. On the contrary to S. mutans and L. rhamnosus, C. albicans has demonstrated the adhesive properties in relation to the above-mentioned surfaces. Moreover, in contrast to S. mutans and C. albicans, L. rhamnosus has not formed the biofilm on the polished IPS Empress material.

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Figures

Figure 1
Figure 1
Dental material used in the study: 1: Vita Enamic; 2: Vita Enamic - polished; 3: IPS Empress Multi; 4: IPS Empress Multi - polished; 5: IPS Empress; 6: IPS Empress - polished; 7: IPS e.max® before sintering; 8: IPS e.max® - polished before sintering; 9: IPS e.max® - after sintering; 10: IPS e.max® - polished after sintering.
Figure 2
Figure 2
A scheme for determining the contact angle Θ of a solid surface.
Figure 3
Figure 3
Samples of dental materials in the suspension of the strain of S. mutans. (sample photo) 1: Vita Enamic; 2: polished Vita Enamic; 3: IPS Empress Multi; 4: polished IPS Empress Multi; 5: IPS Empress; 6: polished IPS Empress.
Figure 4
Figure 4
X-ray powder diffraction patterns of the Vita Enamic.
Figure 5
Figure 5
X-ray powder diffraction of the samples IPS Empress CAD and IPS Empress Multi CAD correlated with COD pattern.
Figure 6
Figure 6
X-ray powder diffraction of the samples IPS e-max CAD and IPS e-max CAD sintered at 800°C correlated with COD pattern.
Figure 7
Figure 7
IR-ATR spectra of the studied materials: (a) hybrid blend and Leucite glass ceramics as well as (b) lithium disilicate glass ceramics.
Figure 8
Figure 8
Macroscopic image of the colony of fungi, streptococci, and bacilli desorbed from the surface of dental materials under the influence of saponin. A: non-polished material; B: polished material.
Figure 9
Figure 9
Quantitative assessment of adhesive properties of the analysed strains in relation to the selected dental materials. All the data in the groups were significantly different in statistical analysis at p≤0.05.
Figure 10
Figure 10
Image of cells on the surface of the tested materials after the use of rhodamine B and acridine orange (AO) obtained from the fluorescent microscope.
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