Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 May 11;18(3):284-289.
doi: 10.1002/rmb2.12273. eCollection 2019 Jul.

Effect on clinical and neonatal outcomes of blastocelic microsuction prior to vitrification

Shingo Mitsuhata  1 , Yuji Endo  1 , Momoko Hayashi  1 , Yoshitaka Fujii  1 , Hiroaki Motoyama  1
Affiliations

Effect on clinical and neonatal outcomes of blastocelic microsuction prior to vitrification

Shingo Mitsuhata et al. Reprod Med Biol. .

Abstract

Purpose: Microsuction (MS) is a technique for mechanically emptying fluid from blastocele using a microneedle. In this study, we evaluated the improvement in clinical and neonatal outcomes of vitrified blastocyst transfer programs when MS of blastocelic fluid was used before vitrification.

Methods: This was a retrospective study based on data collected between March 2014 and August 2016. A total of 317 blastocysts obtained from 211 patients were analyzed. The blastocelic fluid of expanded blastocysts was aspirated completely, and blastocysts were collapsed prior to vitrification. Clinical and neonatal outcomes of warmed blastocysts were compared.

Results: The survival rate of the MS blastocyst was significantly higher compared with the nontreatment control (98.7% vs 89.3%, OR: 9.34, 95% CI: 2.35-36.8, P < 0.01). The rates of implantation and live birth were higher in the MS group than in the control group, but the differences were not significant. There were no differences in gestational age, birthweight, proportion of male babies, rates of cesarean section, and congenital abnormalities.

Conclusion: The MS procedure improved blastocyst survival and had little effect on further embryo development after warming.

Keywords: artificial shrinkage; blastocyst; clinical and neonatal outcomes; microsuction; vitrification.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: Shingo Mitsuhata, Yoshitaka Fujii, Yuji Endo, Momoko Hayashi, and Hiroaki Motoyama declare that they have no conflict of interest. Human rights statement and informed consent: All the procedures were followed in accordance with the ethical standards of the institutional ethical committee and with the Helsinki Declaration of 1964 and its later amendments. All the study's participants provided informed consent, and the study design was approved by the appropriate ethics committee of Kurashiki Medical Clinic, Okayama, Japan. Animal studies: This article does not contain any studies with animal participants performed by any of the authors.

Figures

Figure 1
Figure 1
Microsuction of blastocelic fluid prior to vitrification. A, An expanded blastocyst was immobilized with a holding micropipette. The inner cell mass was located at the 12 o'clock position and the thinnest area of trophectoderm was at the 3 o'clock position. B, The aspirating needle, the same as the needle for intracytoplasmic sperm injection, was positioned at the junction of two trophectoderm cells and slowly inserted into the blastocele. C, The blastocelic fluid was aspirated completely, resulting in collapse of the blastocele. Scale bar = 50μm
See this image and copyright information in PMC

References

    1. Wu CH, Lee TH, Chen HH, Chen CI, Huang CC, Lee MS. The influence of female age on the cumulative live‐birth rate of fresh cycles and subsequent frozen cycles using vitrified blastocysts in hyper‐responders. Taiwan J Obstet Gynecol. 2015;54:567‐571. - PubMed
    1. Kawamura T, Mori M, Arichi A, et al. Elective single embryo transfer: comparison of blastocyst and cleavage‐stage embryo transfer. Reprod Med Biol. 2005;28:197‐201. - PMC - PubMed
    1. Kuramoto T, Boediono A, Egashira A, et al. Selected single blastocyst transfers maintained pregnancy outcome and eliminated multiple pregnancies. Reprod Med Biol. 2004;3:13‐18. - PMC - PubMed
    1. Vladimirov IK, Tacheva D, Diez A. Theory about the embryo cryo‐treatment. Reprod Med Biol. 2017;16:118‐125. - PMC - PubMed
    1. Vanderzwalmen P, Bertin G, Debauche CH, et al. Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. Hum Reprod. 2002;17:744‐751. - PubMed