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. 1988 May 25;110(1):29-36.
doi: 10.1016/0022-1759(88)90079-8.

Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells

C Czerkinsky  1 , G Andersson, H P Ekre, L A Nilsson, L Klareskog, O Ouchterlony
Affiliations

Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells

C Czerkinsky et al. J Immunol Methods. .

Abstract

A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.

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