A suite of solid-state NMR experiments to utilize orphaned magnetization for assignment of proteins using parallel high and low gamma detection

Abstract

We present a suite of two-receiver solid-state NMR experiments for backbone and side chain resonance assignment. The experiments rely on either dipolar coupling or scalar coupling for polarization transfer and are devised to acquire a ¹H-detected 3D experiment AND a nested ¹³C-detected 2D from a shared excitation pulse. In order to compensate for the lower sensitivity of detection on ¹³C nucleus, 2D rows are signal averaged during 3D planes. The 3D dual receiver experiments do not suffer from any appreciable signal loss compared to their single receiver versions and require no extra optimization. The resulting data is higher in information content with no additional experiment time. The approach is expected to become widespread as multiple receivers become standard for new NMR spectrometers.

Keywords: Discarded magnetization pathways; Fast magic angle spinning; Low–γ; Multiple receivers; Protein; Solid-state NMR.