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. 2020 Feb;76(2):645-652.
doi: 10.1002/ps.5561. Epub 2019 Aug 15.

A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population

Jinyi Chen  1 , Zhizhan Chu  2 , Heping Han  1 , Danica E Goggin  1 , Qin Yu  1 , Chad Sayer  3 , Stephen B Powles  1
Affiliations

A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population

Jinyi Chen et al. Pest Manag Sci. 2020 Feb.

Abstract

Background: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population.

Results: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings.

Conlcusion: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population. © 2019 Society of Chemical Industry.

Keywords: Lolium rigidum; dinitroaniline; mutation; resistance; tubulin.

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References

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