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. 2019 Sep 23;63(10):e01166-19.
doi: 10.1128/AAC.01166-19. Print 2019 Oct.

Complete Nucleotide Sequences of mcr-4.3-Carrying Plasmids in Acinetobacter baumannii Sequence Type 345 of Human and Food Origin from the Czech Republic, the First Case in Europe

Ibrahim Bitar  1   2 , Matej Medvecky  3   2 , Tereza Gelbicova  4 , Vladislav Jakubu  5   6 , Jaroslav Hrabak  2 , Helena Zemlickova  5   6 , Renata Karpiskova  4 , Monika Dolejska  3   7
Affiliations

Complete Nucleotide Sequences of mcr-4.3-Carrying Plasmids in Acinetobacter baumannii Sequence Type 345 of Human and Food Origin from the Czech Republic, the First Case in Europe

Ibrahim Bitar et al. Antimicrob Agents Chemother. .

Abstract

Here, we describe two plasmids carrying mcr-4.3 in two Acinetobacter baumannii strains isolated from imported food and a clinical sample. The comparative analysis of these plasmids, with two other plasmids reported in the NCBI database, highlighted the common origin of the plasmidic structure carrying mcr-4.3 This is the first case of the mcr-4.3 gene in a A. baumannii strain isolated from a clinical case in Europe. We hypothesize that food import is initiating the spread in Czech Republic.

Keywords: Acinetobacter baumannii; WGS; colistin resistance; mcr-4.3.

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Figures

FIG 1
FIG 1
Linear map of the four mcr-4.3-carrying plasmids from Acinetobacter baumannii; arrows show the direction of transcription of open reading frames (ORFs), while rectangles represent truncated ORFs. Replication, partitioning genes, mobile elements, mcr-4.3, and other remaining genes are designated by violet, black, yellow, red, and white, respectively. Gray shaded areas represent similar sequences among the respective regions, while dashed lines represent the insertion point of the complex transposon.
See this image and copyright information in PMC

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