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. 2019 Jul 22;6(1):129.
doi: 10.1038/s41597-019-0132-4.

Microbial metagenomes and metatranscriptomes during a coastal phytoplankton bloom

Brent Nowinski  1 , Christa B Smith  1 , Courtney M Thomas  1 , Kaitlin Esson  2   3 , Roman Marin 3rd  4 , Christina M Preston  4 , James M Birch  4 , Christopher A Scholin  4 , Marcel Huntemann  5 , Alicia Clum  5 , Brian Foster  5 , Bryce Foster  5 , Simon Roux  5 , Krishnaveni Palaniappan  5 , Neha Varghese  5 , Supratim Mukherjee  5 , T B K Reddy  5 , Chris Daum  5 , Alex Copeland  5 , I-Min A Chen  5 , Natalia N Ivanova  5 , Nikos C Kyrpides  5 , Tijana Glavina Del Rio  5 , William B Whitman  6 , Ronald P Kiene  2   3 , Emiley A Eloe-Fadrosh  5 , Mary Ann Moran  7
Affiliations

Microbial metagenomes and metatranscriptomes during a coastal phytoplankton bloom

Brent Nowinski et al. Sci Data. .

Abstract

Metagenomic and metatranscriptomic time-series data covering a 52-day period in the fall of 2016 provide an inventory of bacterial and archaeal community genes, transcripts, and taxonomy during an intense dinoflagellate bloom in Monterey Bay, CA, USA. The dataset comprises 84 metagenomes (0.8 terabases), 82 metatranscriptomes (1.1 terabases), and 88 16S rRNA amplicon libraries from samples collected on 41 dates. The dataset also includes 88 18S rRNA amplicon libraries, characterizing the taxonomy of the eukaryotic community during the bloom. Accompanying the sequence data are chemical and biological measurements associated with each sample. These datasets will facilitate studies of the structure and function of marine bacterial communities during episodic phytoplankton blooms.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
MODIS satellite image on September 26, 2016 of the phytoplankton bloom occurring in Monterey Bay and extending into the Pacific. The red dot represents the sampling station M0, located at 36.835 N, 121.901 W.
Fig. 2
Fig. 2
Relative abundance of bacterial and archaeal taxa at Monterey Bay station M0 during the fall of 2016. Samples were collected at ~6 m, and 16S rRNA genes were amplified from community DNA in the 0.22 to 5.0 µm size range. Taxonomic groups were defined based on exact sequence variants using DADA2 in QIIME 2 (https://qiime2.org) and assigned taxonomy with the naive Bayes q2-feature-classifier trained using the 515F/806R region from 99% operational taxonomic units from the SILVA 132 16S rRNA database. Assignments of the 30 most abundant taxa are given at the family level.
Fig. 3
Fig. 3
Relative abundance of eukaryotic taxa at Monterey Bay station M0 during the fall of 2016. Samples were collected at ~6 m, and 18S rRNA genes were amplified from community DNA in the >5.0 µm size range. Taxonomic groups were defined based on exact sequence variants using DADA2 in QIIME 2 (https://qiime2.org) and assigned taxonomy with the naive Bayes q2-feature-classifier trained using the 565F/948R region from 99% operational taxonomic units from the SILVA 132 18S rRNA database.
See this image and copyright information in PMC

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