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. 1988 Jun 6;233(1):74-8.
doi: 10.1016/0014-5793(88)81358-9.

Ca2+ regulation of thyroid NADPH-dependent H2O2 generation

C Dupuy  1 , D Dème, J Kaniewski, J Pommier, A Virion
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Free article

Ca2+ regulation of thyroid NADPH-dependent H2O2 generation

C Dupuy et al. FEBS Lett. .
Free article

Abstract

A thyroid particulate fraction contains an NADPH-dependent H2O2-generating enzyme which requires Ca2+ for activity. A Chaps solubilized extract of the thyroid particulate fraction partially purified by DEAE chromatography did not show a dependence on Ca2+ for activity. Preincubation of the particulate fraction with Ca2+ yielded a preparation insensitive to Ca2+. The non-particulate fraction obtained after incubation of the particles in the presence of Ca2+ was able to inhibit, in the presence of EGTA, the Ca2+-desensitized particulate fraction and the enzyme isolated on DEAE. It is concluded that the reversible Ca2+ activation of the NADPH-dependent H2O2 generation was modulated in porcine thyroid tissue by (a) calcium-releasable inhibitor protein(s).

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