doi: 10.1016/0014-5793(88)80432-0.
Thiol reagents are substrates for the ADP-ribosyltransferase activity of pertussis toxin
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- PMID: 3133246
- DOI: 10.1016/0014-5793(88)80432-0
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Thiol reagents are substrates for the ADP-ribosyltransferase activity of pertussis toxin
FEBS Lett.
.
Free article
Abstract
Thiols such as cysteine and dithiothreitol are substrates for the ADP-ribosyltransferase activity of pertussis toxin. When cysteine was incubated with NAD+ and toxin at pH 7.5, a product containing ADP-ribose and cysteine (presumably ADP-ribosylcysteine) was isolated by high-performance liquid chromatography, and characterized by its composition and release of AMP with phosphodiesterase. Cysteine has a Km of 105 mM at saturating NAD+ concentration. The ability of thiols to act as a substrate is one explanation for the very high concentrations (250 mM or greater) that have been observed to enhance the apparent NAD glycohydrolase activity of the toxin.
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