Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jul;85(13):4700-4.
doi: 10.1073/pnas.85.13.4700.

Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro

K Kawakami  1 , C Scheidereit, R G Roeder
Affiliations

Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro

K Kawakami et al. Proc Natl Acad Sci U S A. 1988 Jul.

Abstract

The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.

PubMed Disclaimer

References

    1. Science. 1987 Nov 27;238(4831):1247-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7881-5 - PubMed
    1. EMBO J. 1987 Dec 1;6(12):3761-70 - PubMed
    1. EMBO J. 1987 Dec 20;6(13):4067-71 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

Publication types

MeSH terms

LinkOut - more resources