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. 2019 Jul 18:52:e20190159.
doi: 10.1590/0037-8682-0159-2019.

Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi

Maisa da Silva Araujo  1   2   3 , Alice Oliveira Andrade  1   2 , Najara Akira Costa Dos Santos  1   2 , Dhélio Batista Pereira  4 , Glaucilene da Silva Costa  1   2 , Paula Frassinetti Medeiros de Paulo  1 , Carlos Tong Rios  5 , Marta Moreno  6   7 , Luiz Hidelbrando Pereira-da-Silva  8 , Jansen Fernandes de Medeiros  1   3
Affiliations
Free article

Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi

Maisa da Silva Araujo et al. Rev Soc Bras Med Trop. .
Free article

Abstract

Introduction: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil.

Methods: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation.

Results: In 11 generations, the colony produced a high number of pupae after the F5 generation.

Conclusions: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.

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