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. 2019 Jul;167(3):311-314.
doi: 10.1007/s10517-019-04516-x. Epub 2019 Jul 26.

Store-Operated Calcium Entry in Mouse Cardiomyocytes

K O Gusev  1 , V V Vigont  2 , D A Grekhnev  2 , A V Shalygin  2 , L N Glushankova  2 , E V Kaznacheeva  2
Affiliations

Store-Operated Calcium Entry in Mouse Cardiomyocytes

K O Gusev et al. Bull Exp Biol Med. 2019 Jul.

Abstract

The fluorescent dye fura-2 AM was employed to record activation of Ca2+ entry in response to a decrease in Ca2+ concentration in the endoplasmic reticulum. Using whole-cell voltage clamp technique, we revealed Ca2+ currents with an amplitude of 0.46±0.13 pA/pF that passed through selective channels with current-voltage characteristics similar to those of classical store-operated CRAC channels. These currents were sensitive to 2-APB (50 μM), an inhibitor of store-operated channels. The data suggest that store-operated calcium entry is a characteristic feature of mature ventricular cardiomyocytes. Pathological alterations in store-operated Ca2+ entry can be implicated in the development of heart diseases.

Keywords: CRAC channels; cardiomyocytes; mouse; store-operated calcium entry.

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