Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019:2030:57-68.
doi: 10.1007/978-1-4939-9639-1_6.

Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope-Resolved Metabolomics Using Ultrahigh-Resolution Mass Spectrometry Coupled with Direct Infusion

Ye Yang  1 , Teresa W-M Fan  2   3 , Andrew N Lane  4   5 , Richard M Higashi  4   5
Affiliations

Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope-Resolved Metabolomics Using Ultrahigh-Resolution Mass Spectrometry Coupled with Direct Infusion

Ye Yang et al. Methods Mol Biol. 2019.

Abstract

Stable isotope-resolved metabolomics (SIRM) is increasingly used among researchers for metabolic studies including amino acid metabolism. However, the classical GC- or HPLC-based methods for amino acid quantification do not meet the needs for multiplexed stable isotope-enriched analysis by ultrahigh-resolution Fourier transform mass spectrometry (UHR-FTMS). This is due to insufficient acquisition time during chromatographic separations and large dynamic range in concentrations of analytes, which compromises detection and quantification of the numerous metabolite isotopologues present in crude extracts. This chapter discusses a modified ethyl chloroformate derivatization method to enable rapid quantitative analysis of stable isotope-enriched amino acids using direct infusion ion introduction coupled with UHR-FTMS.

Keywords: Amino acids; Chloroformate derivatization; Direct infusion nano-electrospray; Stable isotope-resolved metabolomics; Ultrahigh-resolution Fourier transform mass spectrometry.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Reaction scheme of ECF derivatization of amino acids [35].
See this image and copyright information in PMC

References

    1. Rodwell VW, Bender DA, Kennelly PJ, Botham KM, Weil PA Harper’s illustrated biochemistry 30th ed Chp. 29, 30 Lange Medical Books/McGraw-Hill, New York
    1. Yang Y, Fan TW-M, Lane AN, Higashi RM (2017) Chloroformate derivatization for tracing the fate of Amino acids in cells and tissues by multiple stable isotope resolved metabolomics (mSIRM). Anal Chim Acta 976:63–73. doi:10.1016/j.aca.2017.04.014 - DOI - PMC - PubMed
    1. Lane AN, Fan TW-M (2015) Regulation of mammalian nucleotide metabolism and biosynthesis. Nucleic Acids Res 43 (4):2466–2485. doi:10.1093/nar/gkv047 - DOI - PMC - PubMed
    1. Balendiran GK, Dabur R, Fraser D (2004) The role of glutathione in cancer. Cell Biochem Funct 22 (6):343–352. doi:10.1002/cbf.1149 - DOI - PubMed
    1. Chatterjee A, Gupta S (2018) The multifaceted role of glutathione S-transferases in cancer. Cancer Lett 433:33–42. doi:10.1016/j.canlet.2018.06.028 - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources