Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS

Abstract

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Keywords: Amino acid analysis; Amino acid profiling; Fingerprint; HILIC; Mass spectrometry; UPLC-MS.