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. 2019 Jul 7:2019:7481341.
doi: 10.1155/2019/7481341. eCollection 2019.

Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

Letícia Machado Gonçalves  1 , Petrus Levid Barros Madeira  1 , Rafael Soares Diniz  1 , Rammon Farias Nonato  2 , Fabiana Suelen Figuerêdo de Siqueira  1 , Eduardo Martins de Sousa  3 , David Cardoso Sandes Farias  2 , Flaviane Maria Galvão Rocha  3 , Carlos Henrique Lopes Rocha  3 , Andréa Dias Neves Lago  4 , Cristina de Andrade Monteiro  3
Affiliations

Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

Letícia Machado Gonçalves et al. Evid Based Complement Alternat Med. .

Abstract

This study aimed to investigate the effect of the n-butanol fraction of Terminalia catappa Linn., (FBuTC) on biofilm of Candida albicans and Candida glabrata, as well as changes in color and roughness of polymethyl methacrylate resin (PMMA). The susceptibility of C. albicans and C. glabrata to FBuTC was evaluated by means of the Minimum Inhibitory and Minimum Fungicidal Concentration (MIC and MFC). PMMA acrylic resin discs (N= 108) were fabricated. For the susceptibility tests, biofilms of C. albicans and C. glabrata were developed on discs for 48 h and immersed in phosphate-saline buffer solution (PBS), 1% sodium hypochlorite (SH 1%), or FBuTC at MIC, 5xMIC, or 10xMIC. For the color and roughness change tests, the discs were immersed in distilled water, SH 1%, or FBuTC in the concentrations of 0.25 mg/mL, 2.5 mg/mL, or 25.0 mg/mL. After 28 days of incubation, color change was evaluated by spectrophotometry and roughness, by using a profilometer. The biofilms were investigated by one-way ANOVA and, the color and roughness changes (two-way ANOVA and the Tukey test; α=0.05). For both MIC and MFC the value of 0.25 mg/mL of FBuTC was observed for the planktonic cells of C. albicans and C. glabrata. Exposure to FBuTC at 10xMIC had a significant effect on the biofilm of C. albicans, showing a reduction in cell counts when compared with PBS, (p=0.001). For the biofilm of C. glabrata, the MIC was sufficient for significantly reducing the cell count (p<0.001). No important changes in color and roughness of the acrylic resin were observed, even after 28 days, irrespective of the concentration of FBuTC used (p >0.05). It could be concluded that the immersion of acrylic resin for dental prosthesis in FBuTC was effective in reducing the biofilms of C. albicans and C. glabrata without evidence of change in roughness and color of this substrate.

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Figures

Figure 1
Figure 1
Effect of FBuTC on cell counts of biofilm of C. albicans. The presence of different symbols (∗, ∗∗ and ∗∗) indicated statistically significant difference between the groups (one-way ANOVA followed by the Tukey test, p < 0.05).
Figure 2
Figure 2
Effect of FBuTC on cell counts of biofilm of C. glabrata. The presence of different symbols (∗, ∗∗ and ∗) indicated statistically significant difference between the groups (one-way ANOVA followed by the Tukey test, p < 0.05).
Figure 3
Figure 3
Color change values (ΔE) after 28 days of immersion of acrylic resin specimens on FBuTC. The presence of symbol (∗) indicated statistically significant difference between the groups (two-way ANOVA for repeated measures followed by the Tukey test, p < 0.05).
Figure 4
Figure 4
Surface roughness values (ΔRa) after 28 days of immersion of acrylic resin specimens in FBuTC. The presence of symbol (∗) indicated statistically significant difference between the groups (two-way ANOVA for repeated measures followed by the Tukey test, p < 0.05).
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References

    1. Chaves S. C. Oral health in Brazil: the challenges for dental health care models. Brazilian Oral Research. 2012;26(Supplement 1):71–80. doi: 10.1590/S1806-83242012000700011. - DOI - PubMed
    1. Carlsson G. E., Omar R. The future of complete dentures in oral rehabilitation. A critical review. Journal of Oral Rehabilitation. 2010;37(2):143–156. doi: 10.1111/j.1365-2842.2009.02039.x. - DOI - PubMed
    1. Gornitsky M., ParadisI I., Landaverde G., Malo A.-M., Velly A. M. A clinical and microbiological evaluation of denture cleansers for geriatric patients in long-term care institutions. Journal of the Canadian Dental Association. 2002;68(1):39–45. - PubMed
    1. Duyck J., Vandamme K., Krausch-Hofmann S., et al. Impact of denture cleaning method and overnight storage condition on denture biofilm mass and composition: a cross-over randomized clinical trial. PLoS ONE. 2016;11(1) doi: 10.1371/journal.pone.0145837.e0145837 - DOI - PMC - PubMed
    1. Tan C., Tsoi J. K., Seneviratne C. J., Matinlinna J. P. Evaluation of the Candida albicans removal and mechanical properties of denture acrylics cleaned by a low-cost powered toothbrush. Journal of Prosthodontic Research. 2014;58(4):243–251. doi: 10.1016/j.jpor.2014.06.002. - DOI - PubMed