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. 1988 Jul;8(7):2860-8.
doi: 10.1128/mcb.8.7.2860-2868.1988.

Differential stability of c-myc mRNAS in a cell-free system

R Pei  1 , K Calame
Affiliations

Differential stability of c-myc mRNAS in a cell-free system

R Pei et al. Mol Cell Biol. 1988 Jul.

Abstract

We have developed a simple cell-free system for studying the stability of different mRNAs in vitro. We demonstrate that the threefold greater stability in vivo of truncated c-myc mRNA (lacking exon 1) compared with that of full-length c-myc mRNA is maintained in our in vitro system. Chimeric mRNAs in which the first exon of c-myc was fused to immunoglobulin C alpha heavy chain or glyceraldehyde-3-phosphate dehydrogenase mRNAs were not rapidly degraded, demonstrating that c-myc exon 1 alone is not sufficient to tag mRNAs for rapid degradation. Competition experiments show that full-length c-myc mRNA is specifically recognized by a factor(s) responsible for its rapid degradation. This system will allow further characterization and purification of these factors.

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References

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