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. 2020 Jan;287(1):205-217.
doi: 10.1111/febs.15027. Epub 2019 Aug 9.

Formation of adenovirus DNA replication compartments and viral DNA accumulation sites by host chromatin regulatory proteins including NPM1

Michelle Jane Genoveso  1   2 , Miharu Hisaoka  2 , Tetsuro Komatsu  3   4 , Harald Wodrich  3 , Kyosuke Nagata  2 , Mitsuru Okuwaki  2   5
Affiliations
Free article

Formation of adenovirus DNA replication compartments and viral DNA accumulation sites by host chromatin regulatory proteins including NPM1

Michelle Jane Genoveso et al. FEBS J. 2020 Jan.
Free article

Abstract

The adenovirus (Ad) genome is believed to be packaged into the virion by forming a chromatin-like structure. The replicated viral genome is likely to be condensed through binding with viral core proteins before encapsidation. Replicated viral genomes accumulate in the central region of the nucleus, which we termed virus-induced postreplication (ViPR) body. However, the molecular mechanism by which the nuclear structure is reorganized and its functional significance in virus production are currently not understood. In this study, we found that viral packaging protein IVa2, but not capsid proteins, accumulated in the ViPR body. In addition, nucleolar chromatin regulatory proteins, nucleophosmin 1 (NPM1), upstream binding factor, and nucleolin accumulated in the ViPR body in late-stage Ad infection. NPM1 depletion increased the nuclease-resistant viral genome and delayed the ViPR body formation. These results suggested that structural changes in the infected cell nucleus depend on the formation of viral chromatin by host chromatin regulatory proteins. Because NPM1 depletion decreases production of the infectious virion, we propose that host factor-mediated viral chromatin remodeling and concomitant ViPR body formation are prerequisites for efficient encapsidation of Ad chromatin.

Keywords: NPM1; ViPR body; adenovirus; chromatin; nucleolus.

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References

    1. Lee CS, Bishop ES, Zhang R, Yu X, Farina EM, Yan S, Zhao C, Zheng Z, Shu Y, Wu X et al. (2017) Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in the new era of personalized medicine. Genes Dis 4, 43-63.
    1. Chatterjee PK, Vayda ME & Flint SJ (1985) Interactions among the three adenovirus core proteins. J Virol 55, 379-386.
    1. Everitt E, Sundquist B, Pettersson U & Philipson L (1973) Structural proteins of adenoviruses. X. Isolation and topography of low molecular weight antigens from the virion of adenovirus type 2. Virology 52, 130-147.
    1. van Oostrum J & Burnett RM (1985) Molecular composition of the adenovirus type 2 virion. J Virol 56, 439-448.
    1. Nicolas JC, Young CS, Suarez F, Girard M & Levine AJ (1983) Detection, rescue, and mapping of mutations in the adenovirus DNA binding protein gene. Proc Natl Acad Sci USA 80, 1674-1677.

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