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. 2018 Oct-Dec;39(4):226-229.
doi: 10.4103/ayu.AYU_176_16.

Antibacterial and anti-inflammatory properties of Plantago ovata Forssk. leaves and seeds against periodontal pathogens: An in vitro study

P Ravi Tejeshwar Reddy  1 , K V Vandana  1 , Shobha Prakash  1
Affiliations

Antibacterial and anti-inflammatory properties of Plantago ovata Forssk. leaves and seeds against periodontal pathogens: An in vitro study

P Ravi Tejeshwar Reddy et al. Ayu. 2018 Oct-Dec.

Abstract

Background: Plantago commonly called as Isabgol (Plantago ovata Forssk.) is a perennial herb that belongs to the family Plantaginaceae. A range of biological activities has been found from plant extracts, including wound healing activity, anti-inflammatory, analgesic, antioxidant, weak antibiotic, immunomodulating and anti-ulcerogenic activity. Periodontal disease is a complex condition as a result of interaction between microorganisms and host inflammatory mediators. Hence, the extract of Isabgol is tested for its antibacterial and anti-inflammatory properties against periodontal disease.

Aim: The aim of this in vitro study is to evaluate the antibacterial property of Isabgol leaves and seeds against periodontal pathogens, namely Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum and anti-inflammatory property against matrix metalloproteinase-2 (MMP-2) and MMP-9.

Materials and methods: In this in vitro study, aqueous extract of Isabgol is tested for its antibacterial property against the stock cultures of specified periodontal pathogens using the tube dilution method and anti-inflammatory property against MMP-2 and MMP-9 using zymogen gel electrography.

Results: Minimum concentration at which the sensitivity of A. actinomycetemcomitans, P. gingivalis, P. intermedia, and F. nucleatum for the extract observed was 50 μl/ml, 0.8 μl/ml, 0.4 μl/ml and 12.5 μl/ml, respectively, concentrations below these showed no effect on the microorganisms. Zymogen electrographic test for anti-inflammatory activity showed percentage inhibition of 30% and 40% against MMP-2 and MMP-9, respectively.

Conclusion: Isabgol is effective against the periodontal pathogens and inflammatory mediators which are responsible for periodontal disease.

Keywords: Antibacterial; Isabgol; Plantago; anti-inflammatory; matrix metalloproteinases; periodontitis.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Tube dilution method
Figure 2
Figure 2
Zymogen gel electrography. $Matrix metalloproteinase-2, *matrix metalloproteinase-9. 1: Isabgol, 2: Negative control, 3: Positive control
See this image and copyright information in PMC

References

    1. Sharma PK, Chauhan NS, Lal B. Observations on the traditional phytotherapy among the inhabitants of Parvati valley in Western Himalaya, India. J Ethnopharmacol. 2004;92:167–76. - PubMed
    1. Roca-Garcia H. Weeds: A link with the past. Arnoldia. 1972;30:23–4.
    1. Brondegaard V.J. Folk Flora. Kobenhavn: Rosenkilde Bagger; 1987. pp. 68–77.
    1. de Souza AP, Gerlach RF, Line SR. Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts. Dent Mater. 2000;16:103–8. - PubMed
    1. Praveen NC, Rajesh A, Madan M, Chaurasia VR, Hiremath NV, Sharma AM, et al. In vitro evaluation of antibacterial efficacy of pineapple extract (Bromelain) on periodontal pathogens. J Int Oral Health. 2014;6:96–8. - PMC - PubMed