Effects of endogenous and exogenous prostaglandins on glycoprotein synthesis and secretion in isolated rabbit gastric mucosa

Abstract

We studied gastric glycoprotein synthesis and secretion in organ culture before and during cyclooxygenase inhibition and replacement with exogenous prostaglandins (16,16-dimethyl prostaglandin E2 and prostaglandin F2 alpha). Isolated rabbit antral and fundic mucosal explants incorporated [14C]N-acetylglucosamine and [3H]leucine in a linear fashion and steadily secreted labeled proteins and glycoproteins during the 24-h incubation period. On sepharose 4B, greater than 90% of the secreted protein-bound [14C]N-acetylglucosamine was found in the high molecular weight peak. Incorporation of tracer was not influenced by cyclooxygenase inhibition with indomethacin or the addition of exogenous prostaglandins. Secretion of newly formed glycoprotein, however, was significantly inhibited by indomethacin and stimulated by both tested prostaglandins in a concentration-dependent manner. 16,16-Dimethyl prostaglandin E2 caused significant stimulation in concentrations that are well in the physiologic range for endogenous prostaglandin E2, whereas prostaglandin F2 alpha stimulated in 100 times higher concentrations. We conclude that in the isolated gastric mucosa both endogenous and exogenous prostaglandins stimulate mucus secretion. For prostaglandin E2, but not prostaglandin F2 alpha, a role in the physiologic regulation of gastric mucus secretion is probable.