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. 1988 Sep;170(9):4388-91.
doi: 10.1128/jb.170.9.4388-4391.1988.

Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli

P Trieu-Cuot  1 , C Carlier, P Courvalin
Affiliations

Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli

P Trieu-Cuot et al. J Bacteriol. 1988 Sep.

Abstract

The possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pBR322-pAM beta 1 chimeric plasmid, designated pAT191. This shuttle vector, which possesses the tra functions of the streptococcal plasmid pAM beta 1, was conjugatively transferred from Enterococcus faecalis to Escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.

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References

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