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. 2019 Aug 1;186(8):592.
doi: 10.1007/s00604-019-3701-5.

Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles

Hema Bhardwaj  1   2 , Manoj Kumar Pandey  1 , Rajesh  1 , Gajjala Sumana  3   4
Affiliations

Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles

Hema Bhardwaj et al. Mikrochim Acta. .

Abstract

Electrochemical immunosensor for aflatoxin B1 (AFB1) is described that uses a composite prepared from graphene quantum dots (GQDs) and gold nanoparticles (Au NPs). The GQD-AuNP conjugate was obtained by using 2-aminothiophenol (ATP) as a linker where the carboxy groups of GQD bind to the amino groups of crosslinker via conjugation of thiol binding to the AuNP. To evaluate the conjugation of the GQD-AuNP composite, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) was applied. The composite was placed on an indium tin oxide (ITO) electrode and then modified with an antibody against AFB1. By using hexacyanoferrate as the electrochemical probe, the sensor works in the 0.1 to 3.0 ng mL-1 AFB1 concentration range, is highly specific, has good reproducibility and acceptable stability. The biosensor was applied to the analysis of (spiked) maize samples. Conceivably, the method can be utilized to sense other mycotoxins by using their respective antibodies. Graphical abstract Schematic presentation of electrochemical immunosensor for Aflatoxin B1 (AFB1) detection developed by antibodies of AFB1 (anti-AFB1) immobilization on graphene quantum dots (GQDs)-gold nanoparticles (AuNPs) composite deposited by electrophoretic deposition technique on an Indium tin oxide (ITO) surface.

Keywords: Biosensor; Cyclic voltammetry; Electrophoretic deposition; Food toxin; Indium tin oxide.

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References

    1. Wan L, Du C, Yang S (2017) Synthesis of graphene oxide/polybenzoxazine-based nitrogen-containing porous carbon nanocomposite for enhanced supercapacitor properties. Electrochim Acta 251:12–24 - DOI
    1. Rajender G, Giri PK (2016) Formation mechanism of graphene quantum dots and their edge state conversion probed by photoluminescence and Raman spectroscopy. J Mater Chem C 4(46):10852–10865 - DOI
    1. Martin A, Escarpa A (2014) Graphene: the cutting–edge interaction between chemistry and electrochemistry. Trends Anal Chem 56:13–26 - DOI
    1. Mollarasouli F, Asadpour-Zeynali K, Campuzano S, Yáñez-Sedeño P, Pingarrón (2017) Non-enzymatic hydrogen peroxide sensor based on graphene quantum dots-chitosan/methylene blue hybrid nanostructures. Electrochim. Acta 246:303–314
    1. Szabó T, Berkesi O, Forgó P, Josepovits K, Sanakis Y, Petridis D, Dékány I (2006) Evolution of surface functional groups in a series of progressively oxidized graphite oxides. Chem Mater 18(11):2740–2749 - DOI