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. 2019 Aug 1;8(8):263.
doi: 10.3390/antiox8080263.

Antioxidants Profiling of By-Products from Eucalyptus Greenboards Manufacture

Maria Celeiro  1 , J Pablo Lamas  2 , Rosa Arcas  3 , Marta Lores  2
Affiliations

Antioxidants Profiling of By-Products from Eucalyptus Greenboards Manufacture

Maria Celeiro et al. Antioxidants (Basel). .

Abstract

Three different by-products from the Eucalyptus wood industry have been exhaustively characterized to find compounds with antioxidant properties. The industrial process to manufacture Eucalyptus greenboards is distinguished by using just wood and water, which converts the generated by-products in a highly attractive source of bioactive compounds that are originally in the raw material. The studied by-products were: the screw water, derived from the washing of the wood chips; the condensates, obtained after the evaporation and further condensation of the screw water; and finally, the concentrate of eucalyptus. For all of them and for their derived organic extracts, the total polyphenols content (TPC) and antioxidant activity (AA) have been evaluated. The chromatographic fingerprints, based on gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been obtained to identify the main extractable organic wood components. Besides, solid-phase microextraction (SPME) has been employed to characterize the most volatile compounds. Significant differences were observed for the chromatographic profiles of the studied by-products. Up to 48 and 30 different compounds were identified in the screw water, and condensate by-products, respectively; whereas the highest number of compounds, up to 72, have been identified in the organic extracts derived from the concentrate of Eucalyptus, highlighting the presence of monoterpenes, sesquiterpenes, polyphenols, and other bioactive compounds with antioxidant properties. Therefore, these by-products could be exploited to obtain natural extracts with added value which could be reused in the food, cosmetic or pharmaceutical industry, reducing the environmental impact of the industrial activity.

Keywords: analytical characterization; antioxidants; bioactive compounds; wood industry by-products.

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Conflict of interest statement

The authors declare no conflicts of interest

Figures

Figure 1
Figure 1
Outline of the Eucalyptus greenboard production process showing the generated by-products, their derived organic extracts and the chromatographic strategies used for their characterization.
Figure 2
Figure 2
Chromatographic profile obtained for the Eucalyptus screw water. SPME-GC-MS analysis.
Figure 3
Figure 3
Chromatographic profile for the ethanolic (black) and ethyl lactate (red) extracts derived from the screw water. GC-MS analysis. The differences in the retention times are due to the different organic solvents employed.
Figure 4
Figure 4
Chromatographic profile for (a) Condensate 1 (black) and Condensate 2 (red) and (b) detailed characterization for Condensate 1. SPME-GC-MS analysis.
Figure 5
Figure 5
Chromatographic profile obtained for the ethanolic (black) and ethyl lactate (red) extracts derived from the concentrate of Eucalyptus in the (a) polar column and (b) non-polar column.
See this image and copyright information in PMC

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