Silicon dioxide nanoparticles ameliorate the phytotoxic hazards of aluminum in maize grown on acidic soil

Abstract

Aluminum (Al) toxicity is a major constraint for crop production in acid soils. Therefore, looking for sustainable solutions to increase plant tolerance to Al toxicity is needed. Although several studies addressed the potential utilization of silica or silicon dioxide nanoparticles (SNPs) to ameliorate heavy metal phytotoxicity, the exact mechanisms underlying SNPs-induced stress tolerance are still unknown. The current study investigated how SNPs could mitigate Al toxicity in maize plants grown on acidic soil. The impact of Al alone or in combination with SNPs on Al accumulation and detoxification, plant growth, photosynthetic C assimilation and redox homeostasis has been investigated. Al accumulation in stressed-maize organs reduced their growth, decreased photosynthesis related parameters and increased production of reactive oxygen species, through induced NADPH oxidase and photorespiration activities, and cell damage. These effects were more pronounced in roots than in leaves. SNPs ameliorated Al toxicity at growth, physiological and oxidative damage levels. Co-application of SNPs significantly reduced the activities of the photorespiratory enzymes and NADPH oxidase. It stimulated the antioxidant defense systems at enzymatic (superoxide dismutase, catalase, ascorbate and glutathione peroxidases) and non-enzymatic (ascorbate, glutathione, polyphenols, flavonoids, tocopherols, and FRAP) levels. Moreover, SNPs increased organic acids accumulation and metal detoxification (i.e. glutathione-S-transferase activity) in roots, as a protective mechanism against Al toxicity. The SNPs induced-protective mechanisms was dependent on the applied Al concentration and acted in organ-specific manner. Overall, the current study suggests the promising application of SNPs as an innovative approach to mitigate Al phytotoxicity in acidic soils and provides a comprehensive view of the cellular and biochemical mechanisms underlying this mitigation capacity.

Keywords: Aluminum; Metal detoxification; Organ/DoSD-specificity; Redox regulation; Silicon dioxide nanoparticles.