Molecular filtration for recovery of waterborne viruses of fish

Abstract

The effectiveness of tangential flow filtration (TFF) for the recovery of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) from large volumes of water was evaluated. In laboratory studies, virus recovery from IHNV-seeded water following concentration by TFF was approximately 13%. However, the addition of 0.1 and 1% fetal bovine serum to deionized water stabilized the virus, increasing virus recoveries to 95%. The addition of 0.03 and 0.3% beef extract resulted in IHNV recoveries of 80 and 61%, respectively. Similar results were obtained with IPNV-seeded water. Field studies using the TFF procedure were conducted with water from areas where IHNV is endemic. IHNV was detected in effluent from an adult steelhead trout (Salmo gairdneri) holding pond at an estimated concentration of 1 PFU/5 ml of water. It was also detected at levels of 1 PFU/50 ml in water from a 2-m-diameter circular tank containing IHNV-infected steelhead trout fry. IHNV isolated in samples taken from the Metolius River was detected by TFF at estimated levels of 1 PFU/3 liters.