Distribution, Function and Regulation of Type 6 Secretion Systems of Xanthomonadales

Abstract

Members of the Xanthomonadales order include several plant pathogens of significant economic and agricultural impact, such as Xanthomonas spp. Type 6 secretion systems (T6SSs) are contractile nanomachines used by many bacterial species to inject protein effectors into target prokaryotic and eukaryotic cells and provide a competitive advantage for bacteria in different environments. Effectors with antibacterial properties include peptidoglycan hydrolases, lipases and phospholipases that break down structural components of the cell envelope, promoting target-cell lysis; and RNases, DNAses, and NADases that affect target-cell metabolism, arresting growth. Effectors with anti-eukaryotic properties are functionally more diverse. The T6SS of Xanthomonas citri is the only example experimentally characterized so far within the Xanthomonadales order and displays anti-eukaryotic function by providing resistance to predation by amoeba. This T6SS is regulated at the transcriptional level by a signaling cascade involving a Ser/Thr kinase and an extracytoplasmic function (ECF) sigma factor. In this review, we performed in silico analyses of 35 genomes of Xanthomonadales and showed that T6SSs are widely distributed and phylogenetically classified into three major groups. In silico predictions identified a series of proteins with known toxic domains as putative T6SS effectors, suggesting that the T6SSs of Xanthomonadales display both anti-prokaryotic and anti-eukaryotic properties depending on the phylogenetic group and bacterial species.

Keywords: T6SS; Xanthomonadales; Xanthomonas; amoeba predation; bacterial killing; effectors; toxins.

FIGURE 1

Phylogenetic classification of T6SSs within Xanthomonadales and genomic organization of T6SS gene clusters. (A) Phylogenetic distribution of Xanthomonadales T6SS clusters. Maximum-likelihood consensus tree with 1000 bootstrap replicates built with amino acid sequence of TssC (XAC4146) homologs aligned and grouped using MEGA 7.0 (Kumar et al., 2016). (B) Schematic representation of genomic regions encoding the different subtypes of T6SSs.