Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Aug 2;9(8):520.
doi: 10.3390/ani9080520.

Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds

Wei Yan  1 , Xutin Zhao  2 , Juyin Li  2 , Long Cheng  3 , Yanqing Li  4
Affiliations

Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds

Wei Yan et al. Animals (Basel). .

Abstract

Vitronectin plays a role in the blood homeostasis and has been implicated in cell adhesion, migration, and proliferation. Vitronectin has a potential role affecting the residual feed intake (RFI) or feeding efficiency in swine production. Its variations have not been reported in Chinese swine breeds. In this study, two regions of porcine vitronectin were analyzed using PCR and sequencing. The sequence analysis revealed thirteen nucleotide substitutions in region 1 (exon 2- exon 3) and three nucleotide substitutions in region 2 (exon 5- intron 5), which would result in five amino acid changes (p.Ala52Thr, p.Leu94Pro, p.Leu94Gln, p.Gln94Pro, and p.Glu126Gly). In region 1, c.156C/T, c.281A/T, and c.377A/G were the most common (at a total frequency of 49.3%, 31.3% and 31.9% respectively), whereas c.153C/T and c.180C/G were rare (at a total frequency of 1.39%). In region 2, c.597 + 12A/G was the most common (at a total frequency of 39.6%), followed by c.597 + 15A/G (at a total frequency of 31.3%) and c.459A/G (at a total frequency of 16.0%). There was a difference (p < 0.05) in variant frequencies between Chinese breeds and overseas breeds. These results indicate that the porcine vitronectin gene is polymorphic and suggest further analysis is required to see if the variation detected affects RFI or feed efficiency in swines.

Keywords: Vitronectin; feed efficiency; pigs.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Sequencing maps of SNPs. (a) The sequencing map of SNPs (c.153C/T and c.154G/A). (b) The sequencing map of SNP (c.156C/T). (c) The sequencing map of SNP (c.180C/G). (d) The sequencing map of SNP (c.184 + 66C/T). (e) The sequencing map of SNP (c.184 + 81C/T). (f) The sequencing map of SNP (c.189C/T). (g) The sequencing map of SNP (c.281T/C). (h) The sequencing map of SNP (c.281T/A). (i) The sequencing map of SNPs (c.281C/A and c.282A/G). (j) The sequencing map of SNP(c.377A/G). (k) The sequencing map of SNP (c.378 + 5C/T). (l) The sequencing map of SNP (c.459A/G). (m) The sequencing map of SNPs (c.597 + 12A/G and c.597 + 15A/G).
Figure 1
Figure 1
Sequencing maps of SNPs. (a) The sequencing map of SNPs (c.153C/T and c.154G/A). (b) The sequencing map of SNP (c.156C/T). (c) The sequencing map of SNP (c.180C/G). (d) The sequencing map of SNP (c.184 + 66C/T). (e) The sequencing map of SNP (c.184 + 81C/T). (f) The sequencing map of SNP (c.189C/T). (g) The sequencing map of SNP (c.281T/C). (h) The sequencing map of SNP (c.281T/A). (i) The sequencing map of SNPs (c.281C/A and c.282A/G). (j) The sequencing map of SNP(c.377A/G). (k) The sequencing map of SNP (c.378 + 5C/T). (l) The sequencing map of SNP (c.459A/G). (m) The sequencing map of SNPs (c.597 + 12A/G and c.597 + 15A/G).
Figure 1
Figure 1
Sequencing maps of SNPs. (a) The sequencing map of SNPs (c.153C/T and c.154G/A). (b) The sequencing map of SNP (c.156C/T). (c) The sequencing map of SNP (c.180C/G). (d) The sequencing map of SNP (c.184 + 66C/T). (e) The sequencing map of SNP (c.184 + 81C/T). (f) The sequencing map of SNP (c.189C/T). (g) The sequencing map of SNP (c.281T/C). (h) The sequencing map of SNP (c.281T/A). (i) The sequencing map of SNPs (c.281C/A and c.282A/G). (j) The sequencing map of SNP(c.377A/G). (k) The sequencing map of SNP (c.378 + 5C/T). (l) The sequencing map of SNP (c.459A/G). (m) The sequencing map of SNPs (c.597 + 12A/G and c.597 + 15A/G).
Figure 1
Figure 1
Sequencing maps of SNPs. (a) The sequencing map of SNPs (c.153C/T and c.154G/A). (b) The sequencing map of SNP (c.156C/T). (c) The sequencing map of SNP (c.180C/G). (d) The sequencing map of SNP (c.184 + 66C/T). (e) The sequencing map of SNP (c.184 + 81C/T). (f) The sequencing map of SNP (c.189C/T). (g) The sequencing map of SNP (c.281T/C). (h) The sequencing map of SNP (c.281T/A). (i) The sequencing map of SNPs (c.281C/A and c.282A/G). (j) The sequencing map of SNP(c.377A/G). (k) The sequencing map of SNP (c.378 + 5C/T). (l) The sequencing map of SNP (c.459A/G). (m) The sequencing map of SNPs (c.597 + 12A/G and c.597 + 15A/G).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Preissner K.T., Seiffert D. Role of vitronectin and its receptors in haemostasis and vascular remodeling. Thromb. Res. 1998;89:1–21. doi: 10.1016/S0049-3848(97)00298-3. - DOI - PubMed
    1. Ekmekci H., Sonmez H., Ekmekci O.B., Ozturk Z., Domanic N., Kokoglu E. Plasma vitronectin levels in patients with coronary atherosclerosis are increased and correlate with extent of disease. J. Thromb. Thrombolysis. 2002;14:221–225. doi: 10.1023/A:1025000810466. - DOI - PubMed
    1. Bittorf S.V., Williams E.C., Mosher D.F. Alteration of vitronectin. Characterization of changes induced by treatment with urea. J. Biol. Chem. 1993;268:24838–24846. - PubMed
    1. Leavesley D.I., Kashyap A.S., Croll T., Sivaramakrishnan M., Shokoohmand A., Hollier B.G., Upton Z. Vitronectin—Master controller or micromanager. IUBMB Life. 2013;65:807–818. doi: 10.1002/iub.1203. - DOI - PubMed
    1. Zhang D., Hudson A.E., Delostrinos C.F., Carmean N., Eastman R., Jr., Hicks B., Hurst R.E., Bassuk J.A. Dual sources of vitronectin in the human lower urinary tract: Synthesis by urothelium vs. extravasation from the bloodstream. Am. J. Physiol.-Ren. Physiol. 2011;300:475–487. doi: 10.1152/ajprenal.00407.2010. - DOI - PMC - PubMed

LinkOut - more resources