Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Aug 5;20(1):301.
doi: 10.1186/s12882-019-1475-y.

The difference of T cell phenotypes in end stage renal disease patients under different dialysis modality

Jiao Xiaoyan  1   2   3 , Chen Rongyi  1   2   3 , Cao Xuesen  1   2   3 , Zou Jianzhou  1   2   3   4 , Ji Jun  1   2   3   4 , Ding Xiaoqiang  1   2   3   4 , Yu Xiaofang  5   6   7   8
Affiliations

The difference of T cell phenotypes in end stage renal disease patients under different dialysis modality

Jiao Xiaoyan et al. BMC Nephrol. .

Abstract

Background: Impaired T cell immune function exists in end-stage renal disease (ESRD) patients. Dialysis treatment may lead to changes in T cell subsets. In the present study, we aimed to identify alterations of T cell phenotypes in ESRD patients, especially in those receiving peritoneal dialysis (PD), and analyze the potential associated factors.

Methods: In the present study, 110 PD patients and 110 age/gender-matched hemodialysis (HD) patients who met the inclusion criteria were studied. Pre-dialysis blood samples were obtained and analyzed by flow cytometry to detect the expression of CD45RO and CCR7. Univariate and multivariate regression analyses were used to determine the factors associated with the alteration of T cell phenotypes.

Results: In all dialysis patients, age was associated with the frequencies of both CD4+ and CD8+ naïve T cells, effector memory (EM) T cells and effector memory RA (EMRA) T cells but not central memory (CM) T cells. Dialysis modality was also associated with T cell subsets. Compared with HD patients, PD patients showed an increase in both CD4+ and CD8+ CM T cells and a reduction in both CD4+ and CD8+ EM and EMRA T cells. However, the number of CD4+ naïve T cells was lower and the number of CD8+ naïve T cells was higher in PD patients than those in HD patients. In PD patients, further multivariate analysis revealed that the frequency of CD4+ naïve T cells was positively associated with nPCR, while the frequency of CD8+ naïve T cells was negatively associated with age.

Conclusion: In dialysis patients, the dialysis modality and age influence T cell subsets. There is a progression from naïve to effector T cells in HD patients compared with PD patients. In PD patients, different factors may influence the frequencies of CD4+ and CD8+ naïve T cells.

Keywords: End-stage renal disease; Hemodialysis; Peritoneal dialysis; T cell phenotypes.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart of flow cytometry analysis to identify CD4 and CD8 T cells. a The flow cytometry picture of lysed peripheral blood including lymphocytes; b the flow cytometry picture of T cells gated from lymphocytes; c the flow cytometry picture of CD4 T cells and CD8 T cells gated from T cells; d the distribution of naïve T cells (CD45RO − CCR7+), central memory T cells (CM, CD45RO+ CCR7+), effector memory T cells (EM, CD45RO + CCR7−), EMRA (CD45RO − CCR7−) gated from CD4 T cells; e the distribution of naïve T cells (CD45RO − CCR7+), central memory T cells (CM, CD45RO+ CCR7+), effector memory T cells (EM, CD45RO + CCR7−), EMRA (CD45RO − CCR7−) gated from CD8 T cells
See this image and copyright information in PMC

References

    1. Betjes MG, Meijers RW, Litjens NH. Loss of renal function causes premature aging of the immune system. Blood Purif. 2013;36(3–4):173–178. doi: 10.1159/000356084. - DOI - PubMed
    1. Vaziri ND, Pahl MV, Crum A, Norris K. Effect of uremia on structure and function of immune system. J Ren Nutr. 2012;22(1):149–156. doi: 10.1053/j.jrn.2011.10.020. - DOI - PMC - PubMed
    1. Cheung CY, Chan GCW, Chan SK, Ng F, Lam MF, Wong SSH, et al. Cancer incidence and mortality in chronic Dialysis population: a multicenter cohort study. Am J Nephrol. 2016;43(3):153–159. doi: 10.1159/000445362. - DOI - PubMed
    1. Mathew R, Mason D, Kennedy JS. Vaccination issues in patients with chronic kidney disease. Expert Rev Vaccines. 2014;13(2):285–298. doi: 10.1586/14760584.2014.874950. - DOI - PubMed
    1. Appay V, van Lier RA, Sallusto F, Roederer M. Phenotype and function of human T lymphocyte subsets: consensus and issues. Cytometry A. 2008;73(11):975–983. doi: 10.1002/cyto.a.20643. - DOI - PubMed

Publication types

MeSH terms