[Immunochemical comparison of lactoferrin of human milk and lactoferrin of neutrophils]

Abstract

Neutrophilic lactoferrin was isolated from the eluate of the SKN-K hemosorbent utilized for extracorporal blood detoxication. Specific antibodies were isolated from the antiserum obtained after immunization of rabbits using the neutrophilic lactoferrin preparation. Immunological identity of human milk lactoferrin, neutrophilic lactoferrin from blood serum of healthy persons and of the patients with esophagus cancer was exhibited by means of double radial immunodiffusion by Ouchterloni and by double cross immunoelectrophoresis using the antibodies against milk and neutrophilic lactoferrins. Thus, milk lactoferrin, antiserum and antibodies against the enzyme might be used as immunoreagents in studies of neutrophilic lactoferrin.