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. 1988 Sep;265(2):321-8.
doi: 10.1016/0003-9861(88)90134-8.

Generation and isolation of cyanobacterial inside-out thylakoid vesicles

F Nilsson  1 , D J Simpson, A C Stewart, B Andersson
Affiliations

Generation and isolation of cyanobacterial inside-out thylakoid vesicles

F Nilsson et al. Arch Biochem Biophys. 1988 Sep.

Abstract

A method has been designed to prepare inside-out thylakoid vesicles from a cyanobacterial species (Phormidium laminosum). Everted thylakoid vesicles could be generated by Yeda press treatment after induced membrane pairing. Membrane pairing was induced either by addition of high concentrations of Mg2+ ions or by lowering the pH of the fragmentation media. The inside-out vesicles were isolated by aqueous polymer two-phase partition. The membrane orientation was determined by proton translocation studies and freeze-fracture electron microscopy.

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