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. 1988 Jun 15;252(3):753-8.
doi: 10.1042/bj2520753.

Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography

C P Quinn  1 , C C Shone, P C Turnbull, J Melling
Affiliations

Purification of anthrax-toxin components by high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography

C P Quinn et al. Biochem J. .

Abstract

A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than have previously been achieved by conventional chromatographic techniques.

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References

    1. Nature. 1976 Jun 24;261(5562):719-20 - PubMed
    1. Eur J Biochem. 1982 Mar 1;122(3):493-500 - PubMed
    1. Infect Immun. 1983 Jan;39(1):483-6 - PubMed
    1. Methods Enzymol. 1984;104:97-113 - PubMed
    1. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1984;17:189-98 - PubMed

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