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. 1988 Apr;70(4):579-85.
doi: 10.1016/0300-9084(88)90095-8.

Genetic transformation of Streptococcus thermophilus by electroporation

G A Somkuti  1 , D H Steinberg
Affiliations

Genetic transformation of Streptococcus thermophilus by electroporation

G A Somkuti et al. Biochimie. 1988 Apr.

Abstract

A rapid and convenient electroporation procedure was developed for the genetic transformation of intact cells of Streptococcus thermophilus with various species of plasmid DNA. Transformation frequency was influenced by the capacitance and voltage selected for electric pulsing, the pH and composition of the electroporation medium and the molecular mass of the transforming DNA. Electroporation is a simple and effective technique to introduce plasmid DNA into S. thermophilus and useful in the development of recombinant DNA technology for this important industrial microorganism.

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