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. 2019 Aug 9;19(1):348.
doi: 10.1186/s12870-019-1961-1.

Phenotypic and genotypic characterization of near-isogenic lines targeting a major 4BL QTL responsible for pre-harvest sprouting in wheat

Xingyi Wang  1 , Hui Liu  2 , Guannan Liu  1 , Md Sultan Mia  1   3 , Kadambot H M Siddique  1 , Guijun Yan  4
Affiliations

Phenotypic and genotypic characterization of near-isogenic lines targeting a major 4BL QTL responsible for pre-harvest sprouting in wheat

Xingyi Wang et al. BMC Plant Biol. .

Abstract

Background: Resistance to pre-harvest sprouting (PHS) is one of the major objectives in wheat breeding programs. However, the complex quantitative nature of this trait presents challenges when breeding for PHS resistance. Characterization of PHS using near-isogenic lines (NILs) targeting major quantitative trait locus/loci (QTL/QTLs) can be an effective strategy for the identification of responsible genes and underlying mechanisms.

Results: In this study, multiple pairs of NILs were developed and confirmed for a major QTL located on the 4BL chromosome arm that contributes to PHS resistance in wheat, using a combined heterogeneous inbred family method and a fast generation cycling system. Phenotypic characterization of these confirmed NILs revealed significant differences in PHS resistance between the isolines, where the presence of the resistant allele increased the resistance to sprouting on spikes by 54.0-81.9% (average 70.8%) and reduced seed germination by 59.4-70.5% (average 66.2%). The 90 K SNP genotyping assay on the confirmed NIL pairs identified eight SNPs on 4BL, associated with five candidate genes; two of the candidate genes were related to seed dormancy. Agronomic traits in the NIL pairs were investigated; both plant height and grain number per spike were positively correlated with PHS susceptibility.

Conclusions: This study confirmed multiple pairs of NILs and identified SNPs between PHS isolines, which are valuable resources for further fine-mapping of this locus to clone the major genes for PHS resistance and investigate the possible functional regulation of these genes on important agronomic traits, such as plant height and grain number per spike.

Keywords: Grain number per spike; Near-isogenic lines; Plant height; Pre-harvest sprouting; SNP assay; Wheat.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Agarose gel image of PCR products amplified from the parents and the confirmed isolines using maker Xgwm495. The 175 bp-fragments represent the resistant allele, while the 190 bp fragments represent the susceptible allele. M: 100 bp DNA ladder; numbers 1 to 20 refer to the four parents (Chara, Westonia, DM5637B*8 and SUN326AE) and the eight confirmed isoline pairs as indicated in Table 1
Fig. 2
Fig. 2
Spikes and plants of a pair of near-isogenic lines (NIL_PHSR4BL_5). The significant differences between the resistant (on the left of each image) and the susceptible (on the right of each image) isoline was observed in a spike sprouting on day 7, and b plant height
Fig. 3
Fig. 3
Correlation between sprouting percentage and a plant height, and b grain number per spike. All values are means of three replicates from the eight pairs of near-isogenic lines for PHS resistance
Fig. 4
Fig. 4
Differences in agronomic traits in the confirmed isolines with resistant and susceptible alleles. a: plant height, b: grain number per spike and c: yield per plant; ‘*’ and ‘**’ indicate significant differences between the isolines at p < 0.05 and p < 0.01, respectively
See this image and copyright information in PMC

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