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. 2019 Oct 28;58(44):15646-15649.
doi: 10.1002/anie.201904894. Epub 2019 Sep 18.

Peptide Brush Polymers for Efficient Delivery of a Gene Editing Protein to Stem Cells

Angela P Blum  1   2 , David A Nelles  3 , Francisco J Hidalgo  1 , Mollie A Touve  4 , Deborah S Sim  2 , Assael A Madrigal  3 , Gene W Yeo  3   5 , Nathan C Gianneschi  1   4
Affiliations

Peptide Brush Polymers for Efficient Delivery of a Gene Editing Protein to Stem Cells

Angela P Blum et al. Angew Chem Int Ed Engl. .

Abstract

The scarcity of effective means to deliver functional proteins to living cells is a central problem in biotechnology and medicine. Herein, we report the efficient delivery of an active DNA-modifying enzyme to human stem cells through high-density cell penetrating peptide brush polymers. Cre recombinase is mixed with a fluorophore-tagged polymer carrier and then applied directly to induced pluripotent stem cells or HEK293T cells. This results in efficient delivery of Cre protein as measured by activation of a genomically integrated Cre-mediated recombination reporter. We observed that brush polymer formulations utilizing cell penetrating peptides promoted Cre delivery but oligopeptides alone or oligopeptides displayed on nanoparticles did not. Overall, we report the efficient delivery of a genome-modifying enzyme to stem cells that may be generalizable to other, difficult-to-transduce cell types.

Keywords: cell penetrating peptides; cellular uptake; enzyme delivery; protein delivery; stem cells.

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References

    1. K. G. Ford, B. E. Souberbielle, D. Darling, F. Farzaneh, Gene Ther. 2001, 8, 1-4.
    1. P. S. Kabouridis, Trends Biotechnol. 2003, 21, 498-503.
    1. T. K. Kim, J. H. Eberwine, Anal. Bioanal. Chem. 2010, 397, 3173-3178.
    1. M. Zahid, P. D. Robbins, Curr. Gene Ther. 2012, 12, 374-380.
    1. S. Mitragotri, P. A. Burke, R. Langer, Nat. Rev. Drug Discovery 2014, 13, 655-672.

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