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. 2019 Aug 12;9(1):11719.
doi: 10.1038/s41598-019-48239-5.

Construction of a high density linkage map and genome dissection of bruchid resistance in zombi pea (Vigna vexillata (L.) A. Rich)

Kitiya Amkul  1   2   3 , Lixia Wang  4 , Prakit Somta  5   6   7 , Suhua Wang  8 , Xuzhen Cheng  8
Affiliations

Construction of a high density linkage map and genome dissection of bruchid resistance in zombi pea (Vigna vexillata (L.) A. Rich)

Kitiya Amkul et al. Sci Rep. .

Abstract

Zombi pea (Vigna vexillata) is a legume crop that is resistant to several biotic and abiotic stresses. Callosobruchus maculatus and Callosobruchus chinensis are serious stored-insect pests of legume crops. We constructed a high-density linkage map and performed quantitative trait loci (QTLs) mapping for resistance to these insect species in zombi pea. An F2 population of 198 individuals from a cross between 'TVNu 240' (resistant) and 'TVNu 1623' (susceptible) varieties was used to construct a linkage map of 6,529 single nucleotide polymorphism markers generated from sequencing amplified fragments of specific loci. The map comprised 11 linkage groups, spanning 1,740.9 cM, with an average of 593.5 markers per linkage group and an average distance of 0.27 cM between markers. High levels of micro-synteny between V. vexillata and cowpea (Vigna unguiculata), mungbean (Vigna radiata), azuki bean (Vigna angularis) and common bean (Phaseolus vulgaris) were found. One major and three minor QTLs for C. chinensis resistance and one major and one minor QTLs for C. maculatus resistance were identified. The major QTLs for resistance to C. chinensis and C. maculatus appeared to be the same locus. The linkage map developed in this study will facilitate the identification of useful genes/QTLs in zombi pea.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
A high-density genetic linkage map constructed for the Vigna vexillata F2 population of a cross between ‘TVNu 240’ and ‘TVNu1623’ using 6,529 SNP markers from the SLAF sequencing.
Figure 2
Figure 2
Circos map showing genome synteny between the genetic linkage map of Vigna vexillata (LG01–11) and the reference genome sequences of cowpea (V. unguiculata; Vu01–11), mungbean (V. radiata; Vr01–11), azuki bean (V. angualaris; Va01–11), and common bean (Phaseolus vulgaris; Pvu01–11).
Figure 3
Figure 3
Frequency distributions of the percentage of damaged seeds (A) and the area under the disease progress strairs (AUDPS) (B) resulting from C. chinensis and C. maculatus infestation in the Vigna vexillata F2 population of a cross between ‘TVNu 240’ and ‘TVNu1623’.
Figure 4
Figure 4
Linkage group locations of the QTLs identified for the percentage of damaged seeds and the AUDPS resulting from C. chinensis and C. maculatus infestations in the Vigna vexillata F2 population of a cross between ‘TVNu 240’ and ‘TVNu1623’. Bars next to linkages indicate locations of the QTLs based on 1-LOD support. Only partial linkages at QTL sites are shown.
See this image and copyright information in PMC

References

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