Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Apr;35(3):767-775.
doi: 10.1007/s10103-019-02857-7. Epub 2019 Aug 12.

The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis

Zenildo Santos Silva Jr  1   2   3   4 , Cristiane Miranda França  5   6 , Renato Araújo Prates  5   6 , Sergio Brossi Botta  5   6 , Raquel Agnelli Mesquita Ferrari  5 , Patricia Aparecida Ana  7 , Christiane Pavani  5 , Kristianne Porta Santos Fernandes  5   6 , Daniela de Fátima Teixeira da Silva  5 , Michael R Hamblin  8   9   10 , Sandra Kalil Bussadori  11   12
Affiliations

The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis

Zenildo Santos Silva Jr et al. Lasers Med Sci. 2020 Apr.

Abstract

Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds' pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.

Keywords: Cytotoxicity; Dentin; Fourier transform infrared spectroscopy; Papain-based gel.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest

The authors declare non-financial interests concerning the work described. M.R. Hamblin is also a present member of the Transdermal Cap, Inc. scientific advisory board.

Figures

Fig. 1
Fig. 1
Chemical structures of Bixa Orellana (Bixin) and papain
Fig. 2
Fig. 2
Shows the peak positions in the UV-Vis and absorption spectra of the annatto solution extracted from the fruit of the Bixa Orellana (pure Urucum), papain gel (Papacarie Duo), and Papain-gel with Urucum (PapaUrucum)
Fig. 3
Fig. 3
Cytotoxicity—MTT assay with pure Urucum and PapaUrucum
Fig. 4
Fig. 4
Organelles localize mitochondria and lysosomes in live cells, MitoTracker Green applied with PapaUrucum and Hoesch® were used. (A1) PapaUrucum 10 μM. (A2) PapaUrucum 20 μM. (A3) PapaUrucum 30 μM. (A4) PapaUrucum 40 μM. To localize lysosomes, LysoTracher® red was used; for internalization, Papa Urucum™. (B1) Papa Urucum 10 μM. (B2) PapaUrucum 20 μM. (B3) PapaUrucum 30 μM. (B4) PapaUrucum 40 μM
Fig. 5
Fig. 5
(A) Mean infrared spectrum of Urucum (Bixa Orellana extract) obtained in region of 4000 to 600 cm−1. The arrows indicate the main peaks of greater intensity. (B) Mean infrared spectra of control and PapaUrucum obtained in the region of 4000 to 600 cm−1. The arrows indicate the main peaks of greater intensity
Fig. 6
Fig. 6
Mean infrared spectra of type-I collagen membranes treated with Milli-Q water, PapaUrucum, and Papacarie Duo™. (A) Region of 4000 to 600 cm−1; (B) Region of 1800 to 700 cm−1
See this image and copyright information in PMC

References

    1. Bussadori SK, Castro LC, Galvão AC (2006) Papain gel: a new chemo-mechanical caries removal agent. J Clin Pediatr Dent 30(2): 115–119 - PubMed
    1. Bussadori S, Guedes C, Hermida Bruno M, Ram D (2008) Chemo-mechanical removal of caries in an adolescent patient using a pa-pain gel: case report. J Clin Pediatr Dent 32(3):177–180 - PubMed
    1. Motta LJ, Bussadori SK, Campanelli AP, Silva AL, Alfaya TA, Godoy CHL et al. (2014) Randomized controlled clinical trial of long-term chemo-mechanical caries removal using PapacarieTM gel. J Appl Oral Sci 22(4):307–313 - PMC - PubMed
    1. Motta LJ, Bussadori SK, Campanelli AP, Silva AL, Alfaya TA, Godoy CHL et al. (2014) Efficacy of Papacarie¯ in reduction of residual bacteria in deciduous teeth: a randomized, controlled clinical trial. Clinics. 69(5):319–322 - PMC - PubMed
    1. Bohari MR, Chunawalla YK, Ahmed BMN (2012) Clinical evaluation of caries removal in primary teethusing conventional, chemomechanical and lasertechnique: an in vivo study. J Contemp Dent Pract 13(1):40–47 - PubMed

LinkOut - more resources