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. 2019 Aug 13;20(1):644.
doi: 10.1186/s12864-019-6008-3.

Transcriptomic analysis reveals the mechanism of thermosensitive genic male sterility (TGMS) of Brassica napus under the high temperature inducement

Xin Tang  1 , You-Jin Hao  1 , Jun-Xing Lu  1 , Geng Lu  1 , Tao Zhang  2
Affiliations

Transcriptomic analysis reveals the mechanism of thermosensitive genic male sterility (TGMS) of Brassica napus under the high temperature inducement

Xin Tang et al. BMC Genomics. .

Abstract

Background: The thermo-sensitive genic male sterility (TGMS) of Brassica napus facilitates reproductive researches and hybrid seed production. Considering the complexity and little information about the molecular mechanism involved in B. napus TGMS, comparative transcriptomic analyses were peroformed for the sterile (160S-MS) and fertile (160S-MF) flowers to identify potential crucial genes and pathways associated with TGMS.

Results: In total, RNA-seq analysis showed that 2202 genes (561 up-regulated and 1641 down-regulated) were significantly differentially expressed in the fertile flowers of 160S-MF at 25 °C when compared the sterile flower of 160S-MS at 15 °C. Detailed analysis revealed that expression changes in genes encoding heat shock proteins, antioxidant, skeleton protein, GTPase and calmodulin might be involved in TGMS of B. napus. Moreover, gene expression of some key members in plant hormone signaling pathways, such as auxin, gibberellins, jasmonic acid, abscisic acid, brassinosteroid signalings, were significantly surppressed in the flowers of 160S, suggesting that these genes might be involved in the regulation in B. napus TGMS. Here, we also found that transcription factor MADS, NFY, HSF, MYB/C and WRKY might play a crucial role in male fertility under the high temperature condition.

Conclusion: High temperature can significant affect gene expression in the flowers. The findings in the current study improve our understanding of B. napus TGMS at the molecular level and also provide an effective foundation for male fertility researches in other important economic crops.

Keywords: Brassica napus; Differential gene expression; Hormone signaling; Thermo-sensitive genic male sterility; Transcription factor.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Gene function classification of all annotated unigenes by Gene Ontology (GO). The horizontal axis represents the specific GO sub-categories, and the vertical axis represents the number of unigenes
Fig. 2
Fig. 2
KOG functional classification of all mapped unigenes
Fig. 3
Fig. 3
Histogram of cluster of KEGG pathways of assembled unigenes. The horizontal axis is the gene number; and vertical axis is the name of cluster of KEGG. A: Cellular processes; B: Environmental information processing; C: Genetic information processing; D: Metabolism; E: Organismal systems; and F: Cellular Processes
Fig. 4
Fig. 4
qPCR validation of selected genes. The relative expression levels of unigenes were normalized with internal reference gene actin and 18sRNA. Values are means ± SE with three replicated for each samples in qPCR
Fig. 5
Fig. 5
The putative molecular mechanism of male sterility in Brassica napus
See this image and copyright information in PMC

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