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. 1978 Nov 25;20(3):423-33.

Cytochemical and ultrastructural characters of human peripheral blood lymphocytes according to their surface markers

A Desplaces, J Dufer, J Choppin, H Benoist, R T Saracino
  • PMID: 314099

Cytochemical and ultrastructural characters of human peripheral blood lymphocytes according to their surface markers

A Desplaces et al. Nouv Rev Fr Hematol (1978). .

Abstract

The human peripheral blood lymphocytes are characterized by different surface markers: The B lymphocytes by the EAC rosettes and surface immunoglobulin and the T lymphocytes by the E-rosettes. Each lymphoid population has been studied for cytochemical parameters (Acid phosphatase, beta-glucuronidase) by light microscopy and for ultrastructural characteristics by micromanipulation and immunoelectronmicroscopy. It has been shown that the studied enzymes are markers for T cells with high affinity for sheep red blood cells and that the separation of lymphocytes by the rosette techniques leads to homogenous cell populations at the ultrastructural level. A classification of peripheral lymphocytes according to their immunocytochemical profile is discussed.

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