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. 2019 Sep;31(5):792-794.
doi: 10.1177/1040638719871089. Epub 2019 Aug 17.

Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses

Catherine E May  1   2 , Alan J Guthrie  1   2 , Martin L Schulman  1   2
Affiliations

Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses

Catherine E May et al. J Vet Diagn Invest. 2019 Sep.

Abstract

We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis-infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at -20°C for 2-3 y) and 2 from cryopreserved raw semen aliquots (stored at -70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.

Keywords: contagious equine metritis; direct culture–independent strain typing.

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Conflict of interest statement

Declaration of conflicting interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

References

    1. Aalsburg AMet al. Pulsed-field gel electrophoresis genotyping of Taylorella equigenitalis isolates collected in the United States from 1978 to 2010. J Clin Microbiol 2011;49:829–833. - PMC - PubMed
    1. Blacket MJet al. Universal primers for fluorescent labelling of PCR fragments—an efficient and cost-effective approach to genotyping by fluorescence. Mol Ecol Resour 2012;12:456–463. - PubMed
    1. Crowhurst R. Genital infection in mares. Vet Rec 1977;100:476–476. - PubMed
    1. Duquesne Fet al. Development of a single multi-locus sequence typing scheme for Taylorella equigenitalis and Taylorella asinigenitalis. Vet Microbiol 2013;167:609–618. - PubMed
    1. Duquesne Fet al. Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction. Res Vet Sci 2007;82:47–49. - PubMed

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