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. 2019 Aug 16;12(16):2610.
doi: 10.3390/ma12162610.

Toothpaste Composition Effect on Enamel Chromatic and Morphological Characteristics: In Vitro Analysis

Alexandrina Muntean  1 , Sorina Sava  2 , Ada Gabriela Delean  3 , Ana Maria Mihailescu  4 , Laura Silaghi Dumitrescu  5 , Marioara Moldovan  5 , Dana Gabriela Festila  6
Affiliations

Toothpaste Composition Effect on Enamel Chromatic and Morphological Characteristics: In Vitro Analysis

Alexandrina Muntean et al. Materials (Basel). .

Abstract

The aim of this in vitro study was to assess the effect of toothpastes, with different compositions, on optical and morphological features of sound and demineralized enamel. We selected twenty-five teeth, recently extracted for orthodontic purposes, for this in vitro study. The teeth were caries free, without stains, fissures, filling or hypoplasia observed at inspection under standard conditions. Teeth were brushed (for 2-3 min, twice a day, for 21 days), with five different toothpastes (four commercially available and an experimental one) containing fluoride and hydroxyapatite. After that, teeth were demineralized with 37% orthophosforic acid (Ultra Etch®, Ultradent Products Inc., South Jordan, UT, USA) for 60 s. We repeated the brushing protocol for another 21 days on demineralized enamel. Enamel vestibular surfaces were examined using a spectrophotometer (Vita EasyShade -Vita Zahnfabrik, Bad Sackingen, Germany) and a Scanning Electron Microscope (Inspect S®, FEI Company, Hillsboro, OR, USA). Differences were statistically significant for colour parameters L* and ΔE*. SEM evaluation reveals demineralized enamel mineral gain after brushing with selected toothpastes. Toothpastes with specific ingredients can represent a balance between aesthetic and mineralization, and an oral hygiene correct algorithm is able to preserve enamel characteristics during ortodontic treatement with fixed appliances.

Keywords: enamel colour; fluoride; nano hydroxyapatite; toothpaste.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Pre-treatment lateral view of dental arches.
Figure 2
Figure 2
Average values and standard deviations of L* parameter during experimental phases.
Figure 3
Figure 3
Average values and standard deviations of ΔL* during experimental phases.
Figure 4
Figure 4
Average values and standard deviations of b* parameter during experimental phases.
Figure 5
Figure 5
Average values and standard deviations of ΔE* during experimental phases.
Figure 6
Figure 6
Lacalut_es SEM images for sound enamel after T1 (a,d), demineralized enamel surface T2 (b,e), enamel surface after T3 (c,f).
Figure 7
Figure 7
Lacalut_wr SEM images for sound enamel after T1 (a,d), demineralized enamel surface (b,e), enamel surface after T3 (c,f).
Figure 8
Figure 8
Biomed_s SEM images for sound enamel after T1 (a,d), demineralized enamel surface (b,e), enamel surface after T3 (c,f).
Figure 9
Figure 9
Aslamed_st SEM images for sound enamel after T1 (a,d), demineralized enamel surface T2 (b,e), enamel surface after T3 (c,f).
Figure 10
Figure 10
Experim_tp SEM images for sound enamel after T1 (a,d), demineralized enamel surface T2 (b,e), enamel surface after T3 (c,f).
See this image and copyright information in PMC

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