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. 1988 Dec;170(12):5949-52.
doi: 10.1128/jb.170.12.5949-5952.1988.

Analysis of the diphtheria tox promoter by site-directed mutagenesis

J Boyd  1 , J R Murphy
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Analysis of the diphtheria tox promoter by site-directed mutagenesis

J Boyd et al. J Bacteriol. 1988 Dec.

Abstract

By oligonucleotide-directed mutagenesis, we introduced alterations in the two putative -10 regions of the diphtheria tox promoter which are positioned at -50 and -56 from the GUG tox initiation signal. The -10 region positioned at -50 is favored in the expression of ADP-ribosyltransferase activity from the wild-type tox promoter in recombinant Escherichia coli; however, the promoter down mutation at position -50 is compensated for by increased activity of the -10 region positioned at -56.

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