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. 1988 Nov 30;157(1):238-44.
doi: 10.1016/s0006-291x(88)80038-x.

Cloning, sequencing, and expression of cDNA for human beta-galactosidase

A Oshima  1 , A Tsuji, Y Nagao, H Sakuraba, Y Suzuki
Affiliations

Cloning, sequencing, and expression of cDNA for human beta-galactosidase

A Oshima et al. Biochem Biophys Res Commun. .

Abstract

We cloned and sequenced the full-length cDNA for human placental beta-galactosidase. The 2379-nucleotide sequence contains 2031 nucleotides which encode a protein of 677 amino acids. The amino acid sequence includes a putative signal sequence of 23 amino acids and 7 potential asparagine-linked glycosylation sites. The cDNA in the expression vector pSVL was used to transfect COS cells. Expression of the cDNA in transfected COS cells produced immunoprecipitable proteins and led to an increase in beta-galactosidase activity.

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