Electrophysiological and Behavioral Responses of Drosophila suzukii (Diptera: Drosophilidae) Towards the Leaf Volatile β-cyclocitral and Selected Fruit-Ripening Volatiles

Abstract

The spotted-wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), is attracted to numerous volatile organic compounds (VOCs) emitted from several ripening, small fruit crops. However, the strawberry leaf terpenoid, β-cyclocitral, is reportedly very attractive to D. suzukii (but not to all Drosophila spp.). This suggests that leaf and fruit VOCs may be critical sources needed to further develop a more species-specific D. suzukii monitoring lure, or semiochemically based attract-and-kill systems. This study investigated the electrophysiological responses of male and female D. suzukii towards selected host fruit-ripening VOCs and β-cyclocitral, along with behavioral responses toward combined fruit VOCs with β-cyclocitral. Electroantennogram (EAG) results revealed a positive dose-response, for both sexes, as concentrations increased for all VOCs tested. For β-cyclocitral, hexyl acetate and methyl butyrate, the mean male EAG responses were significantly greater than the female responses at lower doses. In caged behavioral bioassays, only in the β-cyclocitral treatments were the mean number of flies captured (sexes combined) significantly greater than that captured in solvent (mineral oil) controls. Our study has demonstrated that while single fruit-based VOCs alone are not enough to cause a strong behavioral attraction by both male and female D. suzukii, the addition of β-cyclocitral did produce a significant attraction response from both sexes. In both bioassays, males had higher responses to β-cyclocitral than females, which suggest a greater sensitivity to this compound. Further studies are needed to ascertain the role β-cyclocitral may play in the development of a more species-specific D. suzukii monitoring lure.

Keywords: attractant; bioassay; electroantennogram; spotted-wing drosophila.