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. 2019 Aug 21;9(9):586.
doi: 10.3390/ani9090586.

Threonine Requirements in Dietary Low Crude Protein for Laying Hens under High-Temperature Environmental Climate

Mahmoud Mostafa Azzam  1   2 , Rashed Alhotan  3 , Abdulaziz Al-Abdullatif  3 , Saud Al-Mufarrej  3 , Mohammed Mabkhot  3 , Ibrahim Abdullah Alhidary  3 , Chuntian Zheng  4
Affiliations

Threonine Requirements in Dietary Low Crude Protein for Laying Hens under High-Temperature Environmental Climate

Mahmoud Mostafa Azzam et al. Animals (Basel). .

Abstract

Lohmann Brown hens (n = 420), at 28 weeks of age, were divided into five dietary treatments, and each treatment included six replicates of 14 laying hens. Dietary crude protein (14%) was presented as the control diet. Dietary L-Thr was added to the control diet for 12 weeks. Dietary Thr levels are 0.43%, 0.49%, 0.57%, 0.66%, and 0.74%, based on digestible base. From 28 to 40 weeks, hen-day egg production presented a quadratic trend to supplementing dietary Thr (R2 = 0.96, p = 0.02), and reached a maximum level at 0.58%. Serum uric acid demonstrated a quadratic trend (R2 = 0.62, p = 0.02) at 0.59%. Both serum total cholesterol and 3-hydroxy-3-methylglutaryl (HMG-CoA) reductase showed lower levels (p < 0.05) at 0.66% Thr. Serum CuZn-SOD elevated (p < 0.05) at 0.49%, 0.57%, and 0.66% Thr, as compared to the control group, and showed a quadratic trend (R2 = 0.87, p = 0.003) at 0.56%. Supplemental L-Thr decreased (p < 0.05) the expression of ileal HSP70 at 0.66% Thr. In summary, the optimal dietary Thr requirements to optimize egg production, serum uric acid, and serum CuZn-SOD were 0.58%, 0.59%, and 0.56%, respectively, by regression analysis.

Keywords: CuZn-SOD; HMG-CoA; HSP70; L-Thr; cholesterol; laying hens.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Average temperature (°C) and humidity (%) inside the experimental farm by week.
Figure 2
Figure 2
Effect of Thr levels on the levels of serum uric acid. Values are means ± standard SEM. Means on each bar with no common letter differ (p < 0.05).
Figure 3
Figure 3
Effect of graded levels of dietary Thr on mRNA expression of ileal HSP70. Values are means ± SEM. means with different superscripts; a,b differ (p < 0.05).
See this image and copyright information in PMC

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