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. 2019 Aug 21;24(17):3035.
doi: 10.3390/molecules24173035.

Green Approach Extraction of Perezone from the Roots of Acourtia platyphilla (A. Grey): A Comparison of Four Activating Modes and Supercritical Carbon Dioxide

René Escobedo-González  1 , Andrea Vázquez Vázquez Cabañas  2 , Armando Martínez González  2 , Pablo Mendoza Sánchez  2 , Zenaida Saavedra-Leos  3 , Julián Cruz-Olivares  4 , Juan Nava Serrano  5 , Joel Martínez  6 , René Miranda Ruvalcaba  7
Affiliations

Green Approach Extraction of Perezone from the Roots of Acourtia platyphilla (A. Grey): A Comparison of Four Activating Modes and Supercritical Carbon Dioxide

René Escobedo-González et al. Molecules. .

Abstract

Perezone, a sesquiterpene quinone, is a very important molecule due to its pharmacological activities in addition to the fact that it is considered to be the first secondary metabolite isolated in the new world (America-Mexico, 1852). This study aims to offer a green comparative study about the extraction of the target molecule from the roots of the vegetable specimen Acourtia platyphilla (A. Grey). The study was performed comparing five different modes of extraction: supercritical CO2, electromagnetic infrared and microwave irradiations, mechanical-wave ultrasound versus typical mantle heating procedure. An exhaustive comparative-discussion of the obtained results is provided. It is worth noting that the corresponding quantifications were established using 1H NMR, correlating appropriately the integrals of the vinylic proton H-6 of perezone with the aromatic singlet of p-dinitrobenzene employed as an internal reference. It is also important to highlight that the four presented procedures are novel modes to extract perezone. Finally, a complementary study about the solubility of the target sesquiterpene quinone related to the use of supercritical CO2 is also reported.

Keywords: comparative extraction; green approach; infrared; microwaves; perezone-sesquiterpene quinone; supercritical carbon dioxide; ultrasound.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Perezone.
Figure 2
Figure 2
Comparative yield extraction percent of perezone for the different treatments employed.
Figure 3
Figure 3
Comparative selectivity extraction percent of perezone for the different treatments employed.
Figure 4
Figure 4
Perezone solubility isotherms in scCO2.
Figure 5
Figure 5
Perezone extraction with scCO2, the yellow crystals correspond to the isolated product.
Figure 6
Figure 6
The experimental conditions tested for the solubility with pressures of 8.06, 9.30, 10.65, and 14.81 MPa with temperatures of 313.15, 323.15, and 333.15 K.
Figure 7
Figure 7
1HNMR of perezone extract with the 1,4 DNB as standard.
See this image and copyright information in PMC

References

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