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. 2019 Aug 1:6:788-794.
doi: 10.1016/j.toxrep.2019.07.011. eCollection 2019.

Hepatoprotective effects of silymarin on CCl4-induced hepatic damage in broiler chickens model

A Baradaran  1 , F Samadi  1 , S S Ramezanpour  2 , S Yousefdoust  1
Affiliations

Hepatoprotective effects of silymarin on CCl4-induced hepatic damage in broiler chickens model

A Baradaran et al. Toxicol Rep. .

Abstract

This study was conducted to investigate the hepatoprotective effects of silymarin on CCl4-induced oxidative stress in broiler chickens model. A total of 240 day-old broilers were divided into 4 equal groups (n = 60) composed of a control group (receiving 1 mL/Kg BW saline) and 3 groups treated with silymarin (receiving 100 mg/Kg BW silymarin), CCl4 (receiving 1 mL/Kg BW CCl4), and combination of silymarin + CCl4. Results indicated that silymarin has potential to mitigate the deleterious effects of CCl4 on protein and lipid metabolism. The protective activity of silymarin against CCl4-mediated lipid peroxidation was demonstrated by the lower serum content of MDA, as lipid peroxidation marker. CCl4-induced hepatotoxicity was demonstrated by the elevation of serum contents of ALP, AST, ALT, and GGT enzymes, whereas silymarin decreased serum activity of ALP and AST hepatic enzymes. The CCl4-challenged birds revealed considerable hepatic injures characterized by moderate to severe hepatocellular degeneration around the portal vein, aggregation of inflammatory cells, granulomatosis, cytolytic necrosis, periportal space fibrosis, and sinusoidal dilatation. However, liver damages were amended by the silymarin. In line with molecular study, a remarkable down-regulation was detected in the expression of CAT, GPx, and Mn-SOD hepatic genes in CCl4-challenged birds, whereas silymarin significantly up-regulated aforementioned genes. In general, current study showed that silymarin has potential to alleviate the adverse effects of oxidative stress in poultry farms.

Keywords: Broiler chickens; CCl4; Gene expression; Oxidative stress; Silymarin.

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Conflict of interest statement

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Figures

Fig. 1
Fig. 1
Photomicrographs of the liver portal triad histopathology in broiler chicks administrated by experimental treatments. A: Control, B: CCl4, C: Silymarin, D: Silymarin + CCl4. Branch of portal vein (PV), branch of portal artery (arrowheads) and branch of bile duct (arrow). Magnification of 20×.
Fig. 2
Fig. 2
The catalase (CAT), glutathione peroxidase (GPx), and manganese-superoxide dismutase (Mn-SOD) relative mRNA expression in the liver of broiler chickens. * P < 0.05, ** P < 0.01 when comparing with the control.
See this image and copyright information in PMC

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